BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0437900 Os10g0437900|Os10g0437900
         (183 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0437900  HSP20-like chaperone domain containing protein      229   8e-61
Os03g0656000  HSP20-like chaperone domain containing protein       70   1e-12

>Os10g0437900 HSP20-like chaperone domain containing protein
          Length = 183

 Score =  229 bits (584), Expect = 8e-61,   Method: Compositional matrix adjust.
 Identities = 124/183 (67%), Positives = 124/183 (67%)

Query: 1   MDQXXXXXXSGSRSRVLSEIDPHSEWIHGDEFDTLVLDVTGFSKDHLKVQVEASGSLRIS 60
           MDQ      SGSRSRVLSEIDPHSEWIHGDEFDTLVLDVTGFSKDHLKVQVEASGSLRIS
Sbjct: 1   MDQAAAAAASGSRSRVLSEIDPHSEWIHGDEFDTLVLDVTGFSKDHLKVQVEASGSLRIS 60

Query: 61  GERAVNGGGRHWLHFLKRFDLPPGAADDASAIKVQLDKGVLYVQVXXXXXXXXXXXXXXX 120
           GERAVNGGGRHWLHFLKRFDLPPGAADDASAIKVQLDKGVLYVQV               
Sbjct: 61  GERAVNGGGRHWLHFLKRFDLPPGAADDASAIKVQLDKGVLYVQVPGPGAPAAAGGSDGE 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXXHPAWQLAGALSRHRQXXXXXXXXXXXXXXXTFANKLT 180
                                  HPAWQLAGALSRHRQ               TFANKLT
Sbjct: 121 QQQQQEGEIAGGRRRAAAARRGGHPAWQLAGALSRHRQVVLNVVIAVVLLWLVTFANKLT 180

Query: 181 KNE 183
           KNE
Sbjct: 181 KNE 183



>Os03g0656000 HSP20-like chaperone domain containing protein
          Length = 292

 Score = 69.7 bits (169), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 37/88 (42%), Positives = 53/88 (60%), Gaps = 6/88 (6%)

Query: 18  SEIDPHSEWIHGDEFDTLVLDVTGFSKDHLKVQVEASGSLRISGERAVNGGGRHWLHFLK 77
           + +DP  EW   ++ DTLV+DV+GF K+ LKV    S  L+++GER  +GG   W  FLK
Sbjct: 21  ANVDPRCEWTRTEDADTLVVDVSGFRKEELKVLYNTSRKLKVAGERRADGG--QWARFLK 78

Query: 78  RFDLPPGAADDASAIKVQLD--KGVLYV 103
            F +P     DA AI+  +D  + +LYV
Sbjct: 79  MFPVPRSC--DAGAIRAVMDNEEALLYV 104



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.134    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,541,938
Number of extensions: 151225
Number of successful extensions: 320
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 319
Number of HSP's successfully gapped: 2
Length of query: 183
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 88
Effective length of database: 12,075,471
Effective search space: 1062641448
Effective search space used: 1062641448
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 153 (63.5 bits)