BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os10g0148000 Os10g0148000|J080001G14
         (115 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os10g0148000  Plant lipid transfer/seed storage/trypsin-alph...   192   6e-50

>Os10g0148000 Plant lipid transfer/seed storage/trypsin-alpha amylase inhibitor
           domain containing protein
          Length = 115

 Score =  192 bits (487), Expect = 6e-50,   Method: Compositional matrix adjust.
 Identities = 96/115 (83%), Positives = 96/115 (83%)

Query: 1   MSQLKSSSXXXXXXXXXXXXXXXXXXXGTECQNDVEVLKTTCYKFVEKDGPKLQPSPDCC 60
           MSQLKSSS                   GTECQNDVEVLKTTCYKFVEKDGPKLQPSPDCC
Sbjct: 1   MSQLKSSSLAALLIFLLAVFTTAAAAAGTECQNDVEVLKTTCYKFVEKDGPKLQPSPDCC 60

Query: 61  TSMKGVNVPCVCTYLGSPGVRDNINMDKVFYVTKQCGIAIPGNCGGEQASLDWPH 115
           TSMKGVNVPCVCTYLGSPGVRDNINMDKVFYVTKQCGIAIPGNCGGEQASLDWPH
Sbjct: 61  TSMKGVNVPCVCTYLGSPGVRDNINMDKVFYVTKQCGIAIPGNCGGEQASLDWPH 115



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.135    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,450,804
Number of extensions: 124756
Number of successful extensions: 202
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 204
Number of HSP's successfully gapped: 1
Length of query: 115
Length of database: 17,035,801
Length adjustment: 82
Effective length of query: 33
Effective length of database: 12,754,253
Effective search space: 420890349
Effective search space used: 420890349
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)