BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os09g0487500 Os09g0487500|AK108131
         (117 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os09g0487500  Conserved hypothetical protein                      177   2e-45
Os08g0505001  Conserved hypothetical protein                      142   4e-35
Os03g0677250                                                       94   2e-20

>Os09g0487500 Conserved hypothetical protein
          Length = 117

 Score =  177 bits (448), Expect = 2e-45,   Method: Compositional matrix adjust.
 Identities = 89/117 (76%), Positives = 89/117 (76%)

Query: 1   MRAISSXXXXXXXXXXXXXXXXXXXXXXXXXXXXWTTPGHEEQPKGYLFNRPPPPAGESR 60
           MRAISS                            WTTPGHEEQPKGYLFNRPPPPAGESR
Sbjct: 1   MRAISSAAGGMLRARLRGGARVRGGGGHGEGGGRWTTPGHEEQPKGYLFNRPPPPAGESR 60

Query: 61  KWEDWELPCYVTSFLTVVILGVGLNAKPDLTLETWAHQKALERLQQQELASGEAQAE 117
           KWEDWELPCYVTSFLTVVILGVGLNAKPDLTLETWAHQKALERLQQQELASGEAQAE
Sbjct: 61  KWEDWELPCYVTSFLTVVILGVGLNAKPDLTLETWAHQKALERLQQQELASGEAQAE 117



>Os08g0505001 Conserved hypothetical protein
          Length = 188

 Score =  142 bits (359), Expect = 4e-35,   Method: Compositional matrix adjust.
 Identities = 65/79 (82%), Positives = 74/79 (93%), Gaps = 2/79 (2%)

Query: 35  WTTPGHEEQPKGYLFNRPPPPAGESRKWEDWELPCYVTSFLTVVILGVGLNAKPDLTLET 94
           WTTPGHEE+PKG+LFNRPPP  GESRKWEDWELPCYVTSFLTV ILGVGL+AKPDLTLET
Sbjct: 17  WTTPGHEERPKGHLFNRPPP--GESRKWEDWELPCYVTSFLTVAILGVGLSAKPDLTLET 74

Query: 95  WAHQKALERLQQQELASGE 113
           WAH KAL+RLQ+++LA+ +
Sbjct: 75  WAHHKALDRLQEKDLAAAD 93



>Os03g0677250 
          Length = 118

 Score = 94.0 bits (232), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 47/73 (64%), Positives = 54/73 (73%), Gaps = 3/73 (4%)

Query: 48  LFNRPPPPAGESRKWEDWELPCYVTSFLTVVILGVGLNAKPDLTLETWAHQKALER---L 104
           LF RP P AGE  KW DWELPCY+TSFLTVVILG+ LNAKP+ TL+T  HQK  ++    
Sbjct: 10  LFKRPSPSAGEFCKWVDWELPCYLTSFLTVVILGIRLNAKPNFTLQTRPHQKERQQATAT 69

Query: 105 QQQELASGEAQAE 117
           +QQEL S EAQ E
Sbjct: 70  EQQELVSMEAQTE 82



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.131    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,896,850
Number of extensions: 157904
Number of successful extensions: 321
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 320
Number of HSP's successfully gapped: 3
Length of query: 117
Length of database: 17,035,801
Length adjustment: 84
Effective length of query: 33
Effective length of database: 12,649,825
Effective search space: 417444225
Effective search space used: 417444225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)