BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os08g0564900 Os08g0564900|AK120585
(100 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os08g0564900 Conserved hypothetical protein 200 2e-52
Os09g0442400 t-snare domain containing protein 91 2e-19
>Os08g0564900 Conserved hypothetical protein
Length = 100
Score = 200 bits (509), Expect = 2e-52, Method: Compositional matrix adjust.
Identities = 100/100 (100%), Positives = 100/100 (100%)
Query: 1 MKWKLLSMVTLLTIAQIKESSTRLVKNHDMLYMPSNLGDLKEVVVHIQRDIKTVGDSMSI 60
MKWKLLSMVTLLTIAQIKESSTRLVKNHDMLYMPSNLGDLKEVVVHIQRDIKTVGDSMSI
Sbjct: 1 MKWKLLSMVTLLTIAQIKESSTRLVKNHDMLYMPSNLGDLKEVVVHIQRDIKTVGDSMSI 60
Query: 61 KMQYLQSTANDIANVVGKSLENHMQLLDGQSKAMVSLINL 100
KMQYLQSTANDIANVVGKSLENHMQLLDGQSKAMVSLINL
Sbjct: 61 KMQYLQSTANDIANVVGKSLENHMQLLDGQSKAMVSLINL 100
>Os09g0442400 t-snare domain containing protein
Length = 639
Score = 90.9 bits (224), Expect = 2e-19, Method: Composition-based stats.
Identities = 48/66 (72%), Positives = 52/66 (78%)
Query: 35 SNLGDLKEVVVHIQRDIKTVGDSMSIKMQYLQSTANDIANVVGKSLENHMQLLDGQSKAM 94
+ + LKE V IQR+IKTVGDSMS KMQ LQSTANDI +V GKSLEN MQLLDGQSKAM
Sbjct: 288 NGMDKLKEEAVDIQREIKTVGDSMSTKMQDLQSTANDIGSVAGKSLENQMQLLDGQSKAM 347
Query: 95 VSLINL 100
L NL
Sbjct: 348 DGLNNL 353
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.317 0.129 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,400,123
Number of extensions: 65470
Number of successful extensions: 205
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 205
Number of HSP's successfully gapped: 2
Length of query: 100
Length of database: 17,035,801
Length adjustment: 69
Effective length of query: 31
Effective length of database: 13,433,035
Effective search space: 416424085
Effective search space used: 416424085
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)