BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0543000 Os08g0543000|Os08g0543000
         (315 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0543000                                                      620   e-178
Os09g0518600  Conserved hypothetical protein                      112   3e-25
Os02g0307300  WD40-like domain containing protein                  82   5e-16

>Os08g0543000 
          Length = 315

 Score =  620 bits (1598), Expect = e-178,   Method: Compositional matrix adjust.
 Identities = 300/315 (95%), Positives = 300/315 (95%)

Query: 1   MAVEENAHAINQSLWFLKMNSIRRFSGMRKPNAPSAIHKDKDESVVFFREMYKREKDRDI 60
           MAVEENAHAINQSLWFLKMNSIRRFSGMRKPNAPSAIHKDKDESVVFFREMYKREKDRDI
Sbjct: 1   MAVEENAHAINQSLWFLKMNSIRRFSGMRKPNAPSAIHKDKDESVVFFREMYKREKDRDI 60

Query: 61  NLLEPMQSVEFDAIQACFRLKTPPATPLFPSIEMETNSSQMVFQKELPIHQQVKPSASRL 120
           NLLEPMQSVEFDAIQACFRLKTPPATPLFPSIEMETNSSQMVFQKELPIHQQVKPSASRL
Sbjct: 61  NLLEPMQSVEFDAIQACFRLKTPPATPLFPSIEMETNSSQMVFQKELPIHQQVKPSASRL 120

Query: 121 SGKTEATKTSARSMFPAPNSSSEKSIFRVSTQSISNEKNQIERRSTSAAITSRKQKVVXX 180
           SGKTEATKTSARSMFPAPNSSSEKSIFRVSTQSISNEKNQIERRSTSAAITSRKQKVV  
Sbjct: 121 SGKTEATKTSARSMFPAPNSSSEKSIFRVSTQSISNEKNQIERRSTSAAITSRKQKVVAA 180

Query: 181 XXXXXXXXXXXXXKKHSERCYASQGSSTNGLKRVTNPELPYCAPKNLITTPLTAKAWRSD 240
                        KKHSERCYASQGSSTNGLKRVTNPELPYCAPKNLITTPLTAKAWRSD
Sbjct: 181 ITPTAPTATCNATKKHSERCYASQGSSTNGLKRVTNPELPYCAPKNLITTPLTAKAWRSD 240

Query: 241 LAFGVHDTAEIGRIRRQSCLPVTVMDGKQKGLPDKVKAVTVSNNRGRAGDATLIKGMRRT 300
           LAFGVHDTAEIGRIRRQSCLPVTVMDGKQKGLPDKVKAVTVSNNRGRAGDATLIKGMRRT
Sbjct: 241 LAFGVHDTAEIGRIRRQSCLPVTVMDGKQKGLPDKVKAVTVSNNRGRAGDATLIKGMRRT 300

Query: 301 DGKKEQRPKHGNQAK 315
           DGKKEQRPKHGNQAK
Sbjct: 301 DGKKEQRPKHGNQAK 315



>Os09g0518600 Conserved hypothetical protein
          Length = 325

 Score =  112 bits (281), Expect = 3e-25,   Method: Compositional matrix adjust.
 Identities = 60/111 (54%), Positives = 71/111 (63%), Gaps = 25/111 (22%)

Query: 38  HKDKDESVVFFREMYKREKDRDINLLEPMQSVEFDAIQACFR------------------ 79
            K++DES+V F E+Y+ EK++D+NLLEPM SVEF+AIQ   R                  
Sbjct: 11  QKNEDESLVLFGELYRHEKEKDVNLLEPMYSVEFEAIQGTSRMFKLPSGKKDYLLPDGGK 70

Query: 80  -----LKTPPATPLFPSIEMETNSSQMVFQKELPIHQQVKPSASRLSGKTE 125
                LKTPPATPLFPS+EME NSSQMVFQ+ELPI Q VK   SR S K E
Sbjct: 71  HDYDWLKTPPATPLFPSLEMEANSSQMVFQRELPILQPVK--TSRFSIKPE 119



>Os02g0307300 WD40-like domain containing protein
          Length = 439

 Score = 82.0 bits (201), Expect = 5e-16,   Method: Compositional matrix adjust.
 Identities = 50/88 (56%), Positives = 57/88 (64%), Gaps = 13/88 (14%)

Query: 201 YASQGSSTNGLKRVTNPELPYCAPKNLITT--PLTAKAWRSDLAFGVHDTAEIGRIRRQS 258
           YASQGS+T+GLK VT PELPY APKNL TT    T +A     A  V +  E GRIRR+S
Sbjct: 59  YASQGSTTSGLKNVTKPELPYNAPKNLTTTRSIFTCRA----PALDVGEKIENGRIRRKS 114

Query: 259 CLPVTV-------MDGKQKGLPDKVKAV 279
           C PV         +DGK+KGLP KVKAV
Sbjct: 115 CPPVATIGTKEQRLDGKKKGLPAKVKAV 142



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.126    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,715,622
Number of extensions: 366043
Number of successful extensions: 808
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 805
Number of HSP's successfully gapped: 3
Length of query: 315
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 214
Effective length of database: 11,762,187
Effective search space: 2517108018
Effective search space used: 2517108018
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 156 (64.7 bits)