BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0540100 Os08g0540100|AK101236
         (381 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0540100  Similar to Biotin synthase (EC 2.8.1.6) (Bioti...   659   0.0  

>Os08g0540100 Similar to Biotin synthase (EC 2.8.1.6) (Biotin synthetase)
          Length = 381

 Score =  659 bits (1699), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 317/337 (94%), Positives = 317/337 (94%)

Query: 45  DGPRNDWSRPEIQAVYDSPLLDLLFHGAQVHRSVHKFREVQQCTLLSIKTGGCSEDCSYC 104
           DGPRNDWSRPEIQAVYDSPLLDLLFHGAQVHRSVHKFREVQQCTLLSIKTGGCSEDCSYC
Sbjct: 45  DGPRNDWSRPEIQAVYDSPLLDLLFHGAQVHRSVHKFREVQQCTLLSIKTGGCSEDCSYC 104

Query: 105 PQSSRYSTGLKAQKLMNKDAVLXXXXXXXXXGSTRFCMGAAWRETIGRKTNFNQILEYVK 164
           PQSSRYSTGLKAQKLMNKDAVL         GSTRFCMGAAWRETIGRKTNFNQILEYVK
Sbjct: 105 PQSSRYSTGLKAQKLMNKDAVLEAAKKAKEAGSTRFCMGAAWRETIGRKTNFNQILEYVK 164

Query: 165 DIRGMGMEVCCTLGMLEKQQAEELKKAGLTAYNHNLDTSREYYPNIISTRSYDDRLQTLE 224
           DIRGMGMEVCCTLGMLEKQQAEELKKAGLTAYNHNLDTSREYYPNIISTRSYDDRLQTLE
Sbjct: 165 DIRGMGMEVCCTLGMLEKQQAEELKKAGLTAYNHNLDTSREYYPNIISTRSYDDRLQTLE 224

Query: 225 HVREAXXXXXXXXXXXLGEAEEDRVGLLHTLATLPAHPESVPINALVAVKGTPLQDQKPV 284
           HVREA           LGEAEEDRVGLLHTLATLPAHPESVPINALVAVKGTPLQDQKPV
Sbjct: 225 HVREAGISICSGGIIGLGEAEEDRVGLLHTLATLPAHPESVPINALVAVKGTPLQDQKPV 284

Query: 285 EIWEMIRMIATARIVMPKAMVRLSAGRVRFSMPEQALCFLAGANSIFAGEKLLTTTNNDF 344
           EIWEMIRMIATARIVMPKAMVRLSAGRVRFSMPEQALCFLAGANSIFAGEKLLTTTNNDF
Sbjct: 285 EIWEMIRMIATARIVMPKAMVRLSAGRVRFSMPEQALCFLAGANSIFAGEKLLTTTNNDF 344

Query: 345 DADQAMFKILGLIPKAPSFGDEAPAADTERCEEAASM 381
           DADQAMFKILGLIPKAPSFGDEAPAADTERCEEAASM
Sbjct: 345 DADQAMFKILGLIPKAPSFGDEAPAADTERCEEAASM 381



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.133    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,683,048
Number of extensions: 394033
Number of successful extensions: 705
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 705
Number of HSP's successfully gapped: 1
Length of query: 381
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 278
Effective length of database: 11,657,759
Effective search space: 3240857002
Effective search space used: 3240857002
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)