BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0526300 Os08g0526300|AK070382
         (329 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0526300  tRNA-binding arm domain containing protein          518   e-147
Os03g0857400  tRNA-binding arm domain containing protein          391   e-109
Os08g0310500  Conserved hypothetical protein                      137   1e-32

>Os08g0526300 tRNA-binding arm domain containing protein
          Length = 329

 Score =  518 bits (1335), Expect = e-147,   Method: Compositional matrix adjust.
 Identities = 261/329 (79%), Positives = 261/329 (79%)

Query: 1   MASLLPSPVFPXXXXXXXXXXXXXXXXXXXXXXXXXXXHGWSNCQGWRLHHRVWAXXXXX 60
           MASLLPSPVFP                           HGWSNCQGWRLHHRVWA     
Sbjct: 1   MASLLPSPVFPTAAAAAASTSSSSRCRCRCRITTSSSAHGWSNCQGWRLHHRVWAAQAAD 60

Query: 61  XXXXXXXXXXXXXXXXXXXXSNVLPYCSINRKEKKTIGEMEQEFLQALQAFYYDKKAVMS 120
                               SNVLPYCSINRKEKKTIGEMEQEFLQALQAFYYDKKAVMS
Sbjct: 61  QQGGVQQQQQQEENEDGVVDSNVLPYCSINRKEKKTIGEMEQEFLQALQAFYYDKKAVMS 120

Query: 121 NEEFDNLKEELMWEGSSVVMLSPDEQRLLEASMAYVAGNPIMTDAEFDELKLRLRKEGSE 180
           NEEFDNLKEELMWEGSSVVMLSPDEQRLLEASMAYVAGNPIMTDAEFDELKLRLRKEGSE
Sbjct: 121 NEEFDNLKEELMWEGSSVVMLSPDEQRLLEASMAYVAGNPIMTDAEFDELKLRLRKEGSE 180

Query: 181 IVQEGPRCSLRSRKVYSDLTVDYFKMFLLNVPXXXXXXXXXXXXXXXXGFEITYLLELPE 240
           IVQEGPRCSLRSRKVYSDLTVDYFKMFLLNVP                GFEITYLLELPE
Sbjct: 181 IVQEGPRCSLRSRKVYSDLTVDYFKMFLLNVPAAVLALTLFFFLDDLTGFEITYLLELPE 240

Query: 241 PFSFIFTWFAALPLIFWVAQAITSAIVKDFLILKGPCPNCGNENLSFFGTILSVPSGGAR 300
           PFSFIFTWFAALPLIFWVAQAITSAIVKDFLILKGPCPNCGNENLSFFGTILSVPSGGAR
Sbjct: 241 PFSFIFTWFAALPLIFWVAQAITSAIVKDFLILKGPCPNCGNENLSFFGTILSVPSGGAR 300

Query: 301 NSVKCANCSSSLVYDSASRLITLPETAEA 329
           NSVKCANCSSSLVYDSASRLITLPETAEA
Sbjct: 301 NSVKCANCSSSLVYDSASRLITLPETAEA 329



>Os03g0857400 tRNA-binding arm domain containing protein
          Length = 324

 Score =  391 bits (1004), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 191/252 (75%), Positives = 213/252 (84%), Gaps = 5/252 (1%)

Query: 81  SNVLPYCSINRK----EKKTIGEMEQEFLQALQAFYYDKKAVMSNEEFDNLKEELMWEGS 136
           SNVL YCSI+ K    EK+++GEMEQEFLQALQAFYYD+KA+MSNEEFDNLKEELMWEGS
Sbjct: 74  SNVLQYCSIDGKGKKAEKRSLGEMEQEFLQALQAFYYDQKAIMSNEEFDNLKEELMWEGS 133

Query: 137 SVVMLSPDEQRLLEASMAYVAGNPIMTDAEFDELKLRLRKEGSEIVQEGPRCSLRSRKVY 196
           SVVMLSPDEQRLLEASMAY AGNPIM+DAEFD+LKLRL+K+GS+IV EGPRCSLRSRKVY
Sbjct: 134 SVVMLSPDEQRLLEASMAYAAGNPIMSDAEFDQLKLRLKKDGSDIVTEGPRCSLRSRKVY 193

Query: 197 SDLTVDYFKMFLLNVPXXXXXXXXXXXXXXXXGFEITYLLELPEPFSFIFTWFAALPLIF 256
           SDLTVDY KMFLLNVP                GFEI  + +LPEPF FIFTWFAALPLI 
Sbjct: 194 SDLTVDYLKMFLLNVPATTLALGLFFFIDELTGFEIN-IFQLPEPFGFIFTWFAALPLIL 252

Query: 257 WVAQAITSAIVKDFLILKGPCPNCGNENLSFFGTILSVPSGGARNSVKCANCSSSLVYDS 316
           ++AQ+IT+AIV DFLILKGPCPNCG ENLSFFGTILSV SGG  N VKCA+CS+ +VYDS
Sbjct: 253 FIAQSITNAIVNDFLILKGPCPNCGAENLSFFGTILSVSSGGETNKVKCADCSTEMVYDS 312

Query: 317 ASRLITLPETAE 328
            SRLITLPE ++
Sbjct: 313 KSRLITLPEPSQ 324



>Os08g0310500 Conserved hypothetical protein
          Length = 199

 Score =  137 bits (344), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 77/144 (53%), Positives = 81/144 (56%), Gaps = 7/144 (4%)

Query: 1   MASLLPSPVFPXXXXXXXXXXXXXXXXXXXXXXXXXXXHGWSNCQGWRLHHRVWAXXXXX 60
           MASLLP PV P                            GWSNCQGWRL HRVWA     
Sbjct: 1   MASLLPRPVLPAAAAASTSSSSRCRYRITTSSSA----RGWSNCQGWRLRHRVWAAHAAD 56

Query: 61  XXXXXXXXXXXXXXXXXXXXSNVLPYCSINRKEKKTIGEMEQEFL--QALQAFYYDKKAV 118
                               SNVLPYCSINRKEKKTIGEM QEFL  +   AFYYDKKA+
Sbjct: 57  QQGGVQQQQQQESEDDVVD-SNVLPYCSINRKEKKTIGEMAQEFLSRRCRPAFYYDKKAI 115

Query: 119 MSNEEFDNLKEELMWEGSSVVMLS 142
           +SNE FDNLKEELMWEGSSVVML+
Sbjct: 116 ISNEVFDNLKEELMWEGSSVVMLT 139



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.135    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,791,123
Number of extensions: 294178
Number of successful extensions: 673
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 661
Number of HSP's successfully gapped: 3
Length of query: 329
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 228
Effective length of database: 11,762,187
Effective search space: 2681778636
Effective search space used: 2681778636
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)