BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0485400 Os08g0485400|AK068155
         (280 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0485400  Similar to 2-nitropropane dioxygenase-like pro...   498   e-141
AK109964                                                           68   8e-12

>Os08g0485400 Similar to 2-nitropropane dioxygenase-like protein
          Length = 280

 Score =  498 bits (1281), Expect = e-141,   Method: Compositional matrix adjust.
 Identities = 250/280 (89%), Positives = 250/280 (89%)

Query: 1   MGWKGILGFDHGVVQAPLGPDISGPELAAAVANAGGIGLLRLPDWPAPDRVRDLIRRTRS 60
           MGWKGILGFDHGVVQAPLGPDISGPELAAAVANAGGIGLLRLPDWPAPDRVRDLIRRTRS
Sbjct: 1   MGWKGILGFDHGVVQAPLGPDISGPELAAAVANAGGIGLLRLPDWPAPDRVRDLIRRTRS 60

Query: 61  LTERPFGAAIVLAFPHEENLRVVLEEKLAVLQVYWGEFPRERVDEAHLAGVKVLHQVGSF 120
           LTERPFGAAIVLAFPHEENLRVVLEEKLAVLQVYWGEFPRERVDEAHLAGVKVLHQVGSF
Sbjct: 61  LTERPFGAAIVLAFPHEENLRVVLEEKLAVLQVYWGEFPRERVDEAHLAGVKVLHQVGSF 120

Query: 121 EEAAKAKEAGVDGIIVQGCEAGGHVIGQEGXXXXXXXXXXXXSDTDISVIAAGGIVDXXX 180
           EEAAKAKEAGVDGIIVQGCEAGGHVIGQEG            SDTDISVIAAGGIVD   
Sbjct: 121 EEAAKAKEAGVDGIIVQGCEAGGHVIGQEGLLPLLPRVVDLVSDTDISVIAAGGIVDGRG 180

Query: 181 XXXXXXXXXXXXXXXTRFLTTEESFAHPLYKKRLIEINCTGYTNVFGRARWPGAPQRVLK 240
                          TRFLTTEESFAHPLYKKRLIEINCTGYTNVFGRARWPGAPQRVLK
Sbjct: 181 YAAALALGAQGVCLGTRFLTTEESFAHPLYKKRLIEINCTGYTNVFGRARWPGAPQRVLK 240

Query: 241 TTFYDQWKNLPEQETEENQPIIGHTIIHGVGYSSVRWYCS 280
           TTFYDQWKNLPEQETEENQPIIGHTIIHGVGYSSVRWYCS
Sbjct: 241 TTFYDQWKNLPEQETEENQPIIGHTIIHGVGYSSVRWYCS 280



>AK109964 
          Length = 589

 Score = 67.8 bits (164), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 65/250 (26%), Positives = 101/250 (40%), Gaps = 22/250 (8%)

Query: 22  ISGPELAAAVANAGGIGLLRLPDWPAPDRVRDLIRRTRSLT--------ERPFGA-AIVL 72
           +  P LAAAV+NAG +G L     P+P+ +R  IR T+SL         E  +G+ A+ +
Sbjct: 24  VGTPALAAAVSNAGALGTLTALTQPSPEALRKAIRETKSLISEDIRKEREGKYGSFAVNI 83

Query: 73  AFPHEEN-------LRVVLEEKLAVLQVYWGEFPRERVDEAHLAGVKVLHQVGSFEEAAK 125
                 N        +  LEE + + +   G  P E +     AG  V+H+  +   A  
Sbjct: 84  TLLPSINPPDYVGYAKAALEEGVRIFETA-GNNPGEVIKVVKAAGAYVIHKCTAVRHARS 142

Query: 126 AKEAGVDGIIVQGCEAGGHVIGQEGXXXXXXXXXXXXSDTDISVIAAGGIVDXXXXXXXX 185
           A+  G D + + G E  GH    E              +  I  IA+GGI +        
Sbjct: 143 AERLGADMLSIDGFECAGH--PGEDDIGGLVLMARAAEELKIPFIASGGIANGQGLASAL 200

Query: 186 XXXXXXXXXXTRFLTTEESFAHPLYKKRLIEINCTGYTNVFGRARWPGAPQRVLKTTFYD 245
                     TRF+ T+ES  H   K+ ++E +    T++F   R      RV      D
Sbjct: 201 ALGAVGANMGTRFMCTKESEIHDNIKRAIVESDERNTTHIFRTLR---NTARVFNDEPRD 257

Query: 246 QWKNLPEQET 255
             ++  +Q T
Sbjct: 258 SNRSSSDQST 267



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.139    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,233,040
Number of extensions: 366342
Number of successful extensions: 793
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 792
Number of HSP's successfully gapped: 2
Length of query: 280
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 180
Effective length of database: 11,814,401
Effective search space: 2126592180
Effective search space used: 2126592180
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 155 (64.3 bits)