BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0478700 Os08g0478700|AK058489
         (65 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0478700  Similar to Mitochondrial uncoupling protein (F...   132   4e-32
Os09g0465400  Mitochondrial substrate carrier family protein      102   6e-23

>Os08g0478700 Similar to Mitochondrial uncoupling protein (Fragment)
          Length = 65

 Score =  132 bits (333), Expect = 4e-32,   Method: Compositional matrix adjust.
 Identities = 65/65 (100%), Positives = 65/65 (100%)

Query: 1  GCSCRGRRRPPYSGAIDCALKTVRSEGVMALYKGFIPTVSRQGPFTVVLFVTLEQVRKVF 60
          GCSCRGRRRPPYSGAIDCALKTVRSEGVMALYKGFIPTVSRQGPFTVVLFVTLEQVRKVF
Sbjct: 1  GCSCRGRRRPPYSGAIDCALKTVRSEGVMALYKGFIPTVSRQGPFTVVLFVTLEQVRKVF 60

Query: 61 NGVEF 65
          NGVEF
Sbjct: 61 NGVEF 65



>Os09g0465400 Mitochondrial substrate carrier family protein
          Length = 321

 Score =  102 bits (254), Expect = 6e-23,   Method: Compositional matrix adjust.
 Identities = 45/56 (80%), Positives = 52/56 (92%)

Query: 10  PPYSGAIDCALKTVRSEGVMALYKGFIPTVSRQGPFTVVLFVTLEQVRKVFNGVEF 65
           PPYSGA+DC +KTVRSEG MALYKGF+PTV+RQGPFT+VLFVTLEQVRK+  GV+F
Sbjct: 266 PPYSGALDCLIKTVRSEGAMALYKGFVPTVTRQGPFTIVLFVTLEQVRKLLKGVDF 321



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.328    0.143    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,142,117
Number of extensions: 57352
Number of successful extensions: 297
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 297
Number of HSP's successfully gapped: 2
Length of query: 65
Length of database: 17,035,801
Length adjustment: 37
Effective length of query: 28
Effective length of database: 15,103,883
Effective search space: 422908724
Effective search space used: 422908724
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 149 (62.0 bits)