BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0457100 Os08g0457100|AK108546
         (130 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0457100  Conserved hypothetical protein                      262   5e-71
Os08g0457200                                                      142   5e-35

>Os08g0457100 Conserved hypothetical protein
          Length = 130

 Score =  262 bits (669), Expect = 5e-71,   Method: Compositional matrix adjust.
 Identities = 130/130 (100%), Positives = 130/130 (100%)

Query: 1   MASFFWPGPPGAQPVGDLQVAKLALLVAGNGFISPSSYLRRCCDDDGPCFVDGAGERCLH 60
           MASFFWPGPPGAQPVGDLQVAKLALLVAGNGFISPSSYLRRCCDDDGPCFVDGAGERCLH
Sbjct: 1   MASFFWPGPPGAQPVGDLQVAKLALLVAGNGFISPSSYLRRCCDDDGPCFVDGAGERCLH 60

Query: 61  LANLVTAMLGLALVLGQMAFFSAAAFRPQVASVTVAARWIVSLAKLATVGTLQLWVYVFC 120
           LANLVTAMLGLALVLGQMAFFSAAAFRPQVASVTVAARWIVSLAKLATVGTLQLWVYVFC
Sbjct: 61  LANLVTAMLGLALVLGQMAFFSAAAFRPQVASVTVAARWIVSLAKLATVGTLQLWVYVFC 120

Query: 121 LCLKMLCIRY 130
           LCLKMLCIRY
Sbjct: 121 LCLKMLCIRY 130



>Os08g0457200 
          Length = 149

 Score =  142 bits (359), Expect = 5e-35,   Method: Compositional matrix adjust.
 Identities = 80/130 (61%), Positives = 92/130 (70%), Gaps = 4/130 (3%)

Query: 3   SFFWPGPPGAQPVGDLQVAKLALLVAGNGFISPSSYLRRC-CDDDGPCFV-DGAGE-RCL 59
           +F WPGPPGAQPVGDL+VA+LALLVAGNG I   SYL  C CDD G CF  DG  E RC 
Sbjct: 2   AFIWPGPPGAQPVGDLRVAQLALLVAGNGLIVSPSYLSSCPCDDGGRCFFQDGVAEQRCF 61

Query: 60  HLANLVTAMLGLALVLGQMAFFSAAAFRP-QVASVTVAARWIVSLAKLATVGTLQLWVYV 118
            LANLVTA+LGLAL++G MAF +A   R  Q  ++   ARW+V LAK+ATVGT Q W+  
Sbjct: 62  DLANLVTAVLGLALLVGDMAFSTAVPGRSTQSQALATVARWLVGLAKVATVGTFQHWINA 121

Query: 119 FCLCLKMLCI 128
           F LCLK   I
Sbjct: 122 FYLCLKTWSI 131



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.330    0.141    0.468 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,955,787
Number of extensions: 202887
Number of successful extensions: 461
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 459
Number of HSP's successfully gapped: 2
Length of query: 130
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 41
Effective length of database: 12,388,755
Effective search space: 507938955
Effective search space used: 507938955
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 150 (62.4 bits)