BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0421700 Os08g0421700|AK107252
         (247 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0421700  Zinc finger, CCHC-type domain containing protein    382   e-106
Os08g0421200                                                       85   6e-17
Os08g0421300                                                       79   3e-15

>Os08g0421700 Zinc finger, CCHC-type domain containing protein
          Length = 247

 Score =  382 bits (982), Expect = e-106,   Method: Compositional matrix adjust.
 Identities = 198/247 (80%), Positives = 198/247 (80%)

Query: 1   MMSSPTXXXXXXXXXXXXXXXYLRWKESVLLRLRTLDLAYVLSEHQPEDGRSDSAKKKRA 60
           MMSSPT               YLRWKESVLLRLRTLDLAYVLSEHQPEDGRSDSAKKKRA
Sbjct: 1   MMSSPTPAPLLPVAPLDGGGGYLRWKESVLLRLRTLDLAYVLSEHQPEDGRSDSAKKKRA 60

Query: 61  RDDELCRGHILATLSDRLLPDYXXXXXXXXXXXXXXXXYDMDGKLPNLPLDRFFAYRFVD 120
           RDDELCRGHILATLSDRLLPDY                YDMDGKLPNLPLDRFFAYRFVD
Sbjct: 61  RDDELCRGHILATLSDRLLPDYAHHATAAAAWRALARTYDMDGKLPNLPLDRFFAYRFVD 120

Query: 121 GEPVLEQLAHLEAMGVAGKLDDRTMYGLVHQKLPPALVKAIALASPPYPDPPSMDHIWDT 180
           GEPVLEQLAHLEAMGVAGKLDDRTMYGLVHQKLPPALVKAIALASPPYPDPPSMDHIWDT
Sbjct: 121 GEPVLEQLAHLEAMGVAGKLDDRTMYGLVHQKLPPALVKAIALASPPYPDPPSMDHIWDT 180

Query: 181 ARLEERMRLCGEIRRGEHTXXXXXXXXXXXXXXXXXXHRSPKRKRRYDRGPCYNCGEPGH 240
           ARLEERMRLCGEIRRGEHT                  HRSPKRKRRYDRGPCYNCGEPGH
Sbjct: 181 ARLEERMRLCGEIRRGEHTQDKEDDDDQGGGDYPDEDHRSPKRKRRYDRGPCYNCGEPGH 240

Query: 241 IARDCRG 247
           IARDCRG
Sbjct: 241 IARDCRG 247



>Os08g0421200 
          Length = 226

 Score = 84.7 bits (208), Expect = 6e-17,   Method: Compositional matrix adjust.
 Identities = 74/246 (30%), Positives = 99/246 (40%), Gaps = 55/246 (22%)

Query: 22  YLRWKESVLLRLRTLDLAYVLSEHQPEDGRSDSAKKKRARDDE----------------- 64
           YLRWKES+LL L T  +A+VLS+  P    + +A   +  D E                 
Sbjct: 16  YLRWKESMLLVLHTAGVAHVLSDEPPPPTPAAAAAGVKEEDGEAEAAAAAARRRWARDDA 75

Query: 65  LCRGHILATLSDRLLPDYXXXXXXXXXXXXXXXXYDMDGKLPNLPLDRFF-AYRFVDGEP 123
           +CRGHILA LSDR+ PDY                YD  G L      R F    F    P
Sbjct: 76  VCRGHILAALSDRIFPDYVRHGTARAAWEAVARAYDGAGALSAGVARRAFDDLEFYANAP 135

Query: 124 VLEQLAHLEAMGVAGK--LDDRTMYGLVHQKLPPALVKAIALASPPYPDPPSMDHIWDTA 181
           +LEQ+AH EA+  A +  L D  + G + + LP ++      AS       +M  +W  A
Sbjct: 136 LLEQIAHAEALNAATRLPLGDEDLAGALCEMLPESVGGP---ASARSGGGATMRDVWRVA 192

Query: 182 RLEERMRLCGEIRRGEHTXXXXXXXXXXXXXXXXXXHRSPKRKRRYDRGPCYNCGEPGHI 241
           RL E  R+C                                R+     G C+ CG+PGH 
Sbjct: 193 RLVETRRVC--------------------------------REDMERHGRCWRCGKPGHH 220

Query: 242 ARDCRG 247
             +C G
Sbjct: 221 TSNCMG 226



>Os08g0421300 
          Length = 222

 Score = 79.3 bits (194), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 61/183 (33%), Positives = 89/183 (48%), Gaps = 20/183 (10%)

Query: 22  YLRWKESVLLRLRTLDLAYVLSEH---------QPEDGRSDSAKKKRARDDELCRGHILA 72
           YLRWKES+LLRL T+ +A+VLS+          + EDG + + ++  ARDD +CRGHILA
Sbjct: 19  YLRWKESMLLRLHTVGVAHVLSDDPPPPPPAGVEEEDGDAAARRRMWARDDAVCRGHILA 78

Query: 73  TLSDRLLPDYXXXXXXXXXXXXXXXXYDMDGKLPNLPLDRFFAYRFVDG--EPVLEQLAH 130
            LSDR+ PDY                YD       +     +    +DG   P+LE++A 
Sbjct: 79  ALSDRIFPDYVRHRTARDAWDAVARTYDNADAASAVAQRMLYDDLALDGAPAPLLERIAR 138

Query: 131 LEAMGVAGK----LDDRTMYGLVHQKLPPALVKAIALASPPYPDPPSMDHIWDTARLEER 186
            EA+  A +    L D  +  L+ Q + PA       A+       +M  +W  AR+ E 
Sbjct: 139 AEALNAATRVTLSLSDAELAELLCQTVLPA-----NAAAAIRSGAATMRDVWRVARIMEA 193

Query: 187 MRL 189
            R+
Sbjct: 194 QRV 196



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.140    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,119,015
Number of extensions: 306692
Number of successful extensions: 1686
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1680
Number of HSP's successfully gapped: 3
Length of query: 247
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 149
Effective length of database: 11,918,829
Effective search space: 1775905521
Effective search space used: 1775905521
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 155 (64.3 bits)