BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0385400 Os08g0385400|J065207L22
         (102 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0385400  Conserved hypothetical protein                       96   5e-21
Os08g0265350  Conserved hypothetical protein                       64   4e-11

>Os08g0385400 Conserved hypothetical protein
          Length = 102

 Score = 96.3 bits (238), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 48/60 (80%), Positives = 48/60 (80%)

Query: 43  LGSAARHPLLTAADNDDDEATLPPPQHRGRGGGSATPRGSGXXXXXXXXXXXXVIWQQVV 102
           LGSAARHPLLTAADNDDDEATLPPPQHRGRGGGSATPRGSG            VIWQQVV
Sbjct: 43  LGSAARHPLLTAADNDDDEATLPPPQHRGRGGGSATPRGSGAADDKAAIALLKVIWQQVV 102



>Os08g0265350 Conserved hypothetical protein
          Length = 142

 Score = 63.5 bits (153), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 32/38 (84%), Positives = 34/38 (89%), Gaps = 1/38 (2%)

Query: 43 LGSAARHPLLTAADNDDDEATLPPPQHRGRGGGSATPR 80
          LGSAARHPLLTAADNDDD+ATLPPPQHRG GG +  PR
Sbjct: 35 LGSAARHPLLTAADNDDDKATLPPPQHRG-GGAARPPR 71



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.131    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,832,956
Number of extensions: 116812
Number of successful extensions: 718
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 716
Number of HSP's successfully gapped: 2
Length of query: 102
Length of database: 17,035,801
Length adjustment: 70
Effective length of query: 32
Effective length of database: 13,380,821
Effective search space: 428186272
Effective search space used: 428186272
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)