BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0374000 Os08g0374000|AK103189
         (151 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0374000  Bet v I allergen family protein                     283   3e-77

>Os08g0374000 Bet v I allergen family protein
          Length = 151

 Score =  283 bits (724), Expect = 3e-77,   Method: Compositional matrix adjust.
 Identities = 139/151 (92%), Positives = 139/151 (92%)

Query: 1   MKSLQGEVHLNIPASKAWEMFTNNETLGKISPEMLSGAEYLEGDGSPGSLRIFKLGPALH 60
           MKSLQGEVHLNIPASKAWEMFTNNETLGKISPEMLSGAEYLEGDGSPGSLRIFKLGPALH
Sbjct: 1   MKSLQGEVHLNIPASKAWEMFTNNETLGKISPEMLSGAEYLEGDGSPGSLRIFKLGPALH 60

Query: 61  HFXXXXXXXXXXXXTGRSFGYEVVSGELKEVYDPYHVTFSFAPVPGKEGEQCVAGWKAEF 120
           HF            TGRSFGYEVVSGELKEVYDPYHVTFSFAPVPGKEGEQCVAGWKAEF
Sbjct: 61  HFVKESVQKVEKVETGRSFGYEVVSGELKEVYDPYHVTFSFAPVPGKEGEQCVAGWKAEF 120

Query: 121 EPITPTSPPPEKAKDAALGFLKLFETCEATN 151
           EPITPTSPPPEKAKDAALGFLKLFETCEATN
Sbjct: 121 EPITPTSPPPEKAKDAALGFLKLFETCEATN 151



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,837,114
Number of extensions: 237538
Number of successful extensions: 484
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 485
Number of HSP's successfully gapped: 1
Length of query: 151
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 59
Effective length of database: 12,232,113
Effective search space: 721694667
Effective search space used: 721694667
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 151 (62.8 bits)