BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0360000 Os08g0360000|AK111240
         (132 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0360000  Conserved hypothetical protein                      256   2e-69
Os03g0852500  Similar to NIR3                                      78   2e-15

>Os08g0360000 Conserved hypothetical protein
          Length = 132

 Score =  256 bits (655), Expect = 2e-69,   Method: Compositional matrix adjust.
 Identities = 125/132 (94%), Positives = 125/132 (94%)

Query: 1   MLATAARWAAKKGKPKMAPIELPAPPEQAQSITRTIFDVVREHGPLTISDVWDHVKDVGL 60
           MLATAARWAAKKGKPKMAPIELPAPPEQAQSITRTIFDVVREHGPLTISDVWDHVKDVGL
Sbjct: 1   MLATAARWAAKKGKPKMAPIELPAPPEQAQSITRTIFDVVREHGPLTISDVWDHVKDVGL 60

Query: 61  RGLTSKRQMKIMMRWMREKQKLRLICDHDGPHKQFLYTTWFTNPKNAPQRPKRDHHRXXX 120
           RGLTSKRQMKIMMRWMREKQKLRLICDHDGPHKQFLYTTWFTNPKNAPQRPKRDHHR   
Sbjct: 61  RGLTSKRQMKIMMRWMREKQKLRLICDHDGPHKQFLYTTWFTNPKNAPQRPKRDHHRQQQ 120

Query: 121 XXXXFRGEPRKP 132
               FRGEPRKP
Sbjct: 121 QQQQFRGEPRKP 132



>Os03g0852500 Similar to NIR3
          Length = 158

 Score = 78.2 bits (191), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 30/74 (40%), Positives = 49/74 (66%)

Query: 25  PPEQAQSITRTIFDVVREHGPLTISDVWDHVKDVGLRGLTSKRQMKIMMRWMREKQKLRL 84
           P E+A +I+  +  +  +HGPLT+ + W+H KD G+ GL SK  MKI+++WM  ++ ++L
Sbjct: 44  PKEEAVAISGRLLQIFTDHGPLTVGNTWNHAKDAGISGLNSKTHMKILLKWMTGRRIVKL 103

Query: 85  ICDHDGPHKQFLYT 98
            C   G  K+FLY+
Sbjct: 104 NCVGTGNSKKFLYS 117



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.135    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,925,273
Number of extensions: 182633
Number of successful extensions: 525
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 525
Number of HSP's successfully gapped: 2
Length of query: 132
Length of database: 17,035,801
Length adjustment: 90
Effective length of query: 42
Effective length of database: 12,336,541
Effective search space: 518134722
Effective search space used: 518134722
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 150 (62.4 bits)