BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0141700 Os08g0141700|AK072310
         (191 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0141700  Pectinesterase inhibitor domain containing pro...   305   2e-83
Os08g0142300                                                       97   7e-21

>Os08g0141700 Pectinesterase inhibitor domain containing protein
          Length = 191

 Score =  305 bits (780), Expect = 2e-83,   Method: Compositional matrix adjust.
 Identities = 156/191 (81%), Positives = 156/191 (81%)

Query: 1   MERSATVFXXXXXXXXXXXXXXXXXXXXXXXPRMSAVEACKQASVGPAMSRTCAETLGTS 60
           MERSATVF                       PRMSAVEACKQASVGPAMSRTCAETLGTS
Sbjct: 1   MERSATVFVALTLLVAGSVVVVVVVDACDGVPRMSAVEACKQASVGPAMSRTCAETLGTS 60

Query: 61  ADEQGATDFMVAAANAATESYKAGKEAVGKVLSNPLAPDGERLPCLVCANKYDDASMLVA 120
           ADEQGATDFMVAAANAATESYKAGKEAVGKVLSNPLAPDGERLPCLVCANKYDDASMLVA
Sbjct: 61  ADEQGATDFMVAAANAATESYKAGKEAVGKVLSNPLAPDGERLPCLVCANKYDDASMLVA 120

Query: 121 STADDAKRCKLSADSLPDLVTAVSAVDECATKMFEESGNTTSVYATAITNRDWXXXXXXX 180
           STADDAKRCKLSADSLPDLVTAVSAVDECATKMFEESGNTTSVYATAITNRDW       
Sbjct: 121 STADDAKRCKLSADSLPDLVTAVSAVDECATKMFEESGNTTSVYATAITNRDWTVLVLRL 180

Query: 181 XXXXXPRQQLS 191
                PRQQLS
Sbjct: 181 ATLVVPRQQLS 191



>Os08g0142300 
          Length = 185

 Score = 97.1 bits (240), Expect = 7e-21,   Method: Compositional matrix adjust.
 Identities = 55/141 (39%), Positives = 75/141 (53%)

Query: 32  PRMSAVEACKQASVGPAMSRTCAETLGTSADEQGATDFMVAAANAATESYKAGKEAVGKV 91
           PRMSAV+ACKQAS    M + C   LG S + +  T F+ AA  A  E+Y    +A  KV
Sbjct: 28  PRMSAVDACKQASTAGVMWQLCVRELGASPEPEEVTGFVAAAMRANREAYGVSYDAAEKV 87

Query: 92  LSNPLAPDGERLPCLVCANKYDDASMLVASTADDAKRCKLSADSLPDLVTAVSAVDECAT 151
             +P +P G       C  KYD A  L+    D    C ++AD   DL +A +AVDECAT
Sbjct: 88  RVDPSSPAGLATVSGYCEGKYDTAQELMTGWIDRLPGCDITADIRVDLASAAAAVDECAT 147

Query: 152 KMFEESGNTTSVYATAITNRD 172
            + +  G  T++Y   + +RD
Sbjct: 148 LLLQNGGEHTTLYQMVLLDRD 168



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.122    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,580,306
Number of extensions: 160319
Number of successful extensions: 418
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 418
Number of HSP's successfully gapped: 4
Length of query: 191
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 96
Effective length of database: 12,075,471
Effective search space: 1159245216
Effective search space used: 1159245216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 153 (63.5 bits)