BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0141300 Os08g0141300|AK067917
         (233 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0141300  Major sperm protein domain containing protein       321   3e-88
Os07g0558800  Major sperm protein domain containing protein       258   3e-69
AK059370                                                          226   1e-59

>Os08g0141300 Major sperm protein domain containing protein
          Length = 233

 Score =  321 bits (822), Expect = 3e-88,   Method: Compositional matrix adjust.
 Identities = 171/233 (73%), Positives = 171/233 (73%)

Query: 1   MPLWGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXVIRSXXXXXXXXXXXXXSKLFFPYEP 60
           MPLWGT                            VIRS             SKLFFPYEP
Sbjct: 1   MPLWGTGSSAASAAAEGSAAGGDGAARSSSGGAAVIRSLLPTRRRLRLDPPSKLFFPYEP 60

Query: 61  GKQVRSAVKIKNISKSHVAFKFQTTAPKSCFMRPPGGILAPGESIIATVFKFVEHPENNE 120
           GKQVRSAVKIKNISKSHVAFKFQTTAPKSCFMRPPGGILAPGESIIATVFKFVEHPENNE
Sbjct: 61  GKQVRSAVKIKNISKSHVAFKFQTTAPKSCFMRPPGGILAPGESIIATVFKFVEHPENNE 120

Query: 121 KPLDQKCKVKFKIVSLKVKGPMEYVPELFDEQKNQVAVEQILRVVFLDAERQTPQMDXXX 180
           KPLDQKCKVKFKIVSLKVKGPMEYVPELFDEQKNQVAVEQILRVVFLDAERQTPQMD   
Sbjct: 121 KPLDQKCKVKFKIVSLKVKGPMEYVPELFDEQKNQVAVEQILRVVFLDAERQTPQMDKLK 180

Query: 181 XXXXXXXXXXXXXXXXXXDTGPRIVGEGLVIDEWKERRERYLARQQIEGVDSA 233
                             DTGPRIVGEGLVIDEWKERRERYLARQQIEGVDSA
Sbjct: 181 RQLAEAEAALEARKKPPEDTGPRIVGEGLVIDEWKERRERYLARQQIEGVDSA 233



>Os07g0558800 Major sperm protein domain containing protein
          Length = 284

 Score =  258 bits (658), Expect = 3e-69,   Method: Compositional matrix adjust.
 Identities = 126/181 (69%), Positives = 146/181 (80%), Gaps = 1/181 (0%)

Query: 52  SKLFFPYEPGKQVRSAVKIKNISKSHVAFKFQTTAPKSCFMRPPGGILAPGESIIATVFK 111
           +KL+FPY+PG+QVRSA++IKN SKSHVAFKFQTTAPKSCFMRPPG ILAPGE+IIATVFK
Sbjct: 104 NKLYFPYQPGQQVRSAIRIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFK 163

Query: 112 FVEHPENNEKPLDQKCKVKFKIVSLKVKGPMEYVPELFDEQKNQVAVEQILRVVFLDAER 171
           FVEHPENNE  L QKCKVKFKI+SLKVKGPM+Y PE+FDEQ++Q  VE+ILRVVFL+ E 
Sbjct: 164 FVEHPENNENVL-QKCKVKFKILSLKVKGPMDYAPEMFDEQRDQAVVEKILRVVFLNVEN 222

Query: 172 QTPQMDXXXXXXXXXXXXXXXXXXXXXDTGPRIVGEGLVIDEWKERRERYLARQQIEGVD 231
             PQ++                     + GP+IVGEGLVIDEWKERRERYLA+QQ+E VD
Sbjct: 223 PGPQLEKLNNQLAEAEAALEARKKPPEENGPKIVGEGLVIDEWKERRERYLAQQQVEVVD 282

Query: 232 S 232
           S
Sbjct: 283 S 283



>AK059370 
          Length = 171

 Score =  226 bits (576), Expect = 1e-59,   Method: Compositional matrix adjust.
 Identities = 112/162 (69%), Positives = 127/162 (78%), Gaps = 1/162 (0%)

Query: 71  KNISKSHVAFKFQTTAPKSCFMRPPGGILAPGESIIATVFKFVEHPENNEKPLDQKCKVK 130
           +N SKSHVAFKFQTTAPKSCFMRPPG ILAPGE+IIATVFKFVEHPENNE  L QKCKVK
Sbjct: 10  QNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEHPENNENVL-QKCKVK 68

Query: 131 FKIVSLKVKGPMEYVPELFDEQKNQVAVEQILRVVFLDAERQTPQMDXXXXXXXXXXXXX 190
           FKI+SLKVKGPM+Y PE+FDEQ++Q  VE+ILRVVFL+ E   PQ++             
Sbjct: 69  FKILSLKVKGPMDYAPEMFDEQRDQAVVEKILRVVFLNVENPGPQLEKLNNQLAEAEAAL 128

Query: 191 XXXXXXXXDTGPRIVGEGLVIDEWKERRERYLARQQIEGVDS 232
                   + GP+IVGEGLVIDEWKERRERYLA+QQ+E VDS
Sbjct: 129 EARKKPPEENGPKIVGEGLVIDEWKERRERYLAQQQVEVVDS 170



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,694,590
Number of extensions: 188124
Number of successful extensions: 402
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 402
Number of HSP's successfully gapped: 3
Length of query: 233
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 135
Effective length of database: 11,918,829
Effective search space: 1609041915
Effective search space used: 1609041915
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 154 (63.9 bits)