BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0135900 Os08g0135900|AK072535
         (471 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0135900  Similar to Tryptophan synthase beta chain 1 (E...   846   0.0  
AB003491                                                          718   0.0  

>Os08g0135900 Similar to Tryptophan synthase beta chain 1 (EC 4.2.1.20) (Orange
           pericarp 1) (Fragment)
          Length = 471

 Score =  846 bits (2186), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 409/409 (100%), Positives = 409/409 (100%)

Query: 63  GEVNGAASGGFARPDAFGRFGKFGGKYVPETLMHALTELEAAFHALAGDEDFQKELDGIL 122
           GEVNGAASGGFARPDAFGRFGKFGGKYVPETLMHALTELEAAFHALAGDEDFQKELDGIL
Sbjct: 63  GEVNGAASGGFARPDAFGRFGKFGGKYVPETLMHALTELEAAFHALAGDEDFQKELDGIL 122

Query: 123 KDYVGRETPLYFAERLTEHYKRADGTGPMIYLKREDLNHTGAHKINNAVAQVLLAKRLGK 182
           KDYVGRETPLYFAERLTEHYKRADGTGPMIYLKREDLNHTGAHKINNAVAQVLLAKRLGK
Sbjct: 123 KDYVGRETPLYFAERLTEHYKRADGTGPMIYLKREDLNHTGAHKINNAVAQVLLAKRLGK 182

Query: 183 ERIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGT 242
           ERIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGT
Sbjct: 183 ERIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGT 242

Query: 243 ATLKDATSEAIRDWVTNVENTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMEKWGGK 302
           ATLKDATSEAIRDWVTNVENTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMEKWGGK
Sbjct: 243 ATLKDATSEAIRDWVTNVENTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMEKWGGK 302

Query: 303 PDVLVACVGGGSNAMGLFHEFVDDQDIRMIGVEAAGYGVDTDKHAATLTKGEVGVLHGSL 362
           PDVLVACVGGGSNAMGLFHEFVDDQDIRMIGVEAAGYGVDTDKHAATLTKGEVGVLHGSL
Sbjct: 303 PDVLVACVGGGSNAMGLFHEFVDDQDIRMIGVEAAGYGVDTDKHAATLTKGEVGVLHGSL 362

Query: 363 SYVLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLE 422
           SYVLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLE
Sbjct: 363 SYVLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLE 422

Query: 423 GIIPALETSHALAYLEKLCPTLPDGVRVVVNCSGRGDKDVHTASKYLDV 471
           GIIPALETSHALAYLEKLCPTLPDGVRVVVNCSGRGDKDVHTASKYLDV
Sbjct: 423 GIIPALETSHALAYLEKLCPTLPDGVRVVVNCSGRGDKDVHTASKYLDV 471



>AB003491 
          Length = 470

 Score =  718 bits (1853), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 347/398 (87%), Positives = 369/398 (92%), Gaps = 2/398 (0%)

Query: 76  PDAFGRFGKFGGKYVPETLMHALTELEAAFHALAGDEDFQKELDGILKDYVGRETPLYFA 135
           PD+FGRFG F GKYV ETLMHAL+ELE+AF  LA D+DFQ+EL GILKDYVGR +PLYF 
Sbjct: 73  PDSFGRFGTFQGKYVGETLMHALSELESAFKKLATDDDFQRELAGILKDYVGRLSPLYFV 132

Query: 136 ERLTEHYKRADGTGPMIYLKREDLNHTGAHKINNAVAQVLLAKRLGKERIIAETGAGQHG 195
           E LTEHY+R +GTGP+IYLKRED NHTGAHKINNAVAQ L AKRLGK+RIIAETGAGQHG
Sbjct: 133 ESLTEHYRRENGTGPLIYLKREDNNHTGAHKINNAVAQALSAKRLGKKRIIAETGAGQHG 192

Query: 196 VATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGTATLKDATSEAIRD 255
           VATATVCA FGL+CIIYMGAQDMERQALNVFRMKLLGAEVR VHSGTATLKDATSEAIRD
Sbjct: 193 VATATVCASFGLECIIYMGAQDMERQALNVFRMKLLGAEVREVHSGTATLKDATSEAIRD 252

Query: 256 WVTNVENTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMEKWGGKPDVLVACVGGGSN 315
           WVTNVENTHYILGSVAGPHPYPMMVREFHKVIGKETRRQA+EKWGGKPDVLVACVGGGSN
Sbjct: 253 WVTNVENTHYILGSVAGPHPYPMMVREFHKVIGKETRRQALEKWGGKPDVLVACVGGGSN 312

Query: 316 AMGLFHEFVDDQDIRMIGVEAAGYGVDTDKHAATLTKGEVGVLHGSL--SYVLQDDDGQV 373
           AMGLFHEFVDDQ++RMIGVEAAG+GVD+ KHAATLTKG+VGVLHGSL  SY+LQDDDGQV
Sbjct: 313 AMGLFHEFVDDQEVRMIGVEAAGFGVDSVKHAATLTKGDVGVLHGSLAMSYLLQDDDGQV 372

Query: 374 IEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLEGIIPALETSHA 433
           IEPHSISAGLDYPGVGPEHSF+KD+GRAEYDS TD+EAL  FKRVSRLEGIIPALETSHA
Sbjct: 373 IEPHSISAGLDYPGVGPEHSFVKDMGRAEYDSATDEEALAGFKRVSRLEGIIPALETSHA 432

Query: 434 LAYLEKLCPTLPDGVRVVVNCSGRGDKDVHTASKYLDV 471
           LAYLEKLCPTL DG RVV N SGRGDKDV T +K LDV
Sbjct: 433 LAYLEKLCPTLFDGTRVVFNFSGRGDKDVDTVAKSLDV 470



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,886,520
Number of extensions: 662980
Number of successful extensions: 1713
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1712
Number of HSP's successfully gapped: 2
Length of query: 471
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 367
Effective length of database: 11,605,545
Effective search space: 4259235015
Effective search space used: 4259235015
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)