BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os08g0127800 Os08g0127800|AK103321
         (257 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os08g0127800  Conserved hypothetical protein                      152   2e-37

>Os08g0127800 Conserved hypothetical protein
          Length = 257

 Score =  152 bits (384), Expect = 2e-37,   Method: Compositional matrix adjust.
 Identities = 79/116 (68%), Positives = 79/116 (68%)

Query: 1   MSCFGMASGSSAGWPLXXXXXXXXXXXXXXXKPWRLWWRRCAGVAAVIQSKIHRRAVRWP 60
           MSCFGMASGSSAGWPL               KPWRLWWRRCAGVAAVIQSKIHRRAVRWP
Sbjct: 1   MSCFGMASGSSAGWPLSPSPSSTRGRRRVSVKPWRLWWRRCAGVAAVIQSKIHRRAVRWP 60

Query: 61  XXXXXXXXXXXXXXXXXXXXXXWCHHRSFAPVYVDELYSHPKTHHVAVHEAQAQQP 116
                                 WCHHRSFAPVYVDELYSHPKTHHVAVHEAQAQQP
Sbjct: 61  GGHGGGRRRREAATASTREREGWCHHRSFAPVYVDELYSHPKTHHVAVHEAQAQQP 116




 Score =  108 bits (270), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 53/53 (100%), Positives = 53/53 (100%)

Query: 205 RSLLMSPLRGGGACGMGEVDVRAEVFIRKFREEMRLQNQKSAEEFQAMLARGL 257
           RSLLMSPLRGGGACGMGEVDVRAEVFIRKFREEMRLQNQKSAEEFQAMLARGL
Sbjct: 205 RSLLMSPLRGGGACGMGEVDVRAEVFIRKFREEMRLQNQKSAEEFQAMLARGL 257



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.134    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,473,176
Number of extensions: 104637
Number of successful extensions: 414
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 412
Number of HSP's successfully gapped: 2
Length of query: 257
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 158
Effective length of database: 11,866,615
Effective search space: 1874925170
Effective search space used: 1874925170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 155 (64.3 bits)