BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0692800 Os07g0692800|Os07g0692800
         (338 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0692800  Similar to Eukaryotic translation initiation f...   613   e-176
Os03g0296400  Similar to Eukaryotic translation initiation f...   479   e-136

>Os07g0692800 Similar to Eukaryotic translation initiation factor 2 subunit 1
           (Eukaryotic translation initiation factor 2 alpha
           subunit) (eIF-2-alpha) (EIF- 2alpha) (EIF-2A) (Fragment)
          Length = 338

 Score =  613 bits (1581), Expect = e-176,   Method: Compositional matrix adjust.
 Identities = 303/338 (89%), Positives = 303/338 (89%)

Query: 1   MPNLECRMYEAPFPEVETAVMIQVKHLAELGAYVSLLEYNNIEGMXXXXXXXXXXXXXXX 60
           MPNLECRMYEAPFPEVETAVMIQVKHLAELGAYVSLLEYNNIEGM               
Sbjct: 1   MPNLECRMYEAPFPEVETAVMIQVKHLAELGAYVSLLEYNNIEGMILYSELSRRRIRSIP 60

Query: 61  XXXKVGRQEPAVVLRVDHDKGYIDLSKRRVSHHDRRTCEDRYSKSKFVHSIMRHLAETLH 120
              KVGRQEPAVVLRVDHDKGYIDLSKRRVSHHDRRTCEDRYSKSKFVHSIMRHLAETLH
Sbjct: 61  SLIKVGRQEPAVVLRVDHDKGYIDLSKRRVSHHDRRTCEDRYSKSKFVHSIMRHLAETLH 120

Query: 121 LDLEPLYHRIAWPLYRTYGHAFDAFKLIVADPDAAILDSLTYDLTETGPDDQEVTKTLPA 180
           LDLEPLYHRIAWPLYRTYGHAFDAFKLIVADPDAAILDSLTYDLTETGPDDQEVTKTLPA
Sbjct: 121 LDLEPLYHRIAWPLYRTYGHAFDAFKLIVADPDAAILDSLTYDLTETGPDDQEVTKTLPA 180

Query: 181 VTPEIKDALIKNIRRRMTPQPHKIRADIDMKCFQYDGVLHIQEAMRKAEAAGNKDCPVKI 240
           VTPEIKDALIKNIRRRMTPQPHKIRADIDMKCFQYDGVLHIQEAMRKAEAAGNKDCPVKI
Sbjct: 181 VTPEIKDALIKNIRRRMTPQPHKIRADIDMKCFQYDGVLHIQEAMRKAEAAGNKDCPVKI 240

Query: 241 KLVAAPLYVLTTETLDKHQGISVLNNAIKACGETIEKHKGKLVVKEAPRAVSEREDRLFM 300
           KLVAAPLYVLTTETLDKHQGISVLNNAIKACGETIEKHKGKLVVKEAPRAVSEREDRLFM
Sbjct: 241 KLVAAPLYVLTTETLDKHQGISVLNNAIKACGETIEKHKGKLVVKEAPRAVSEREDRLFM 300

Query: 301 DDIEKLKIANXXXXXXXXXXXXXXXXXVDLTKTGVGSQ 338
           DDIEKLKIAN                 VDLTKTGVGSQ
Sbjct: 301 DDIEKLKIANEEVDGDEDSEEDTGMGDVDLTKTGVGSQ 338



>Os03g0296400 Similar to Eukaryotic translation initiation factor 2 subunit 1
           (Eukaryotic translation initiation factor 2 alpha
           subunit) (eIF-2-alpha) (EIF- 2alpha) (EIF-2A) (Fragment)
          Length = 339

 Score =  479 bits (1234), Expect = e-136,   Method: Compositional matrix adjust.
 Identities = 238/339 (70%), Positives = 266/339 (78%), Gaps = 3/339 (0%)

Query: 1   MPNLECRMYEAPFPEVETAVMIQVKHLAELGAYVSLLEYNNIEGMXXXXXXXXXXXXXXX 60
           MPNLECRMYE  FPEV+ AVMIQVKH+A++GAYVSLLEYNNIEGM               
Sbjct: 1   MPNLECRMYEPRFPEVDAAVMIQVKHIADMGAYVSLLEYNNIEGMILFSELSRRRIRSIS 60

Query: 61  XXXKVGRQEPAVVLRVDHDKGYIDLSKRRVSHHDRRTCEDRYSKSKFVHSIMRHLAETLH 120
              KVGRQEPA+VLRVD DKGYIDLSKRRVS  +   CEDRY+KSK VHSIMRH+AETL 
Sbjct: 61  SLIKVGRQEPAIVLRVDRDKGYIDLSKRRVSEEEAHACEDRYNKSKLVHSIMRHVAETLG 120

Query: 121 LDLEPLYHRIAWPLYRTYGHAFDAFKLIVADPDAAILDSLTYDLTETGPDDQEVTKTLPA 180
           +DLEPLY RI WPLYR YGHAF+AFKLIVADPDA ILDSLTY+  ETG D QEVTK +PA
Sbjct: 121 IDLEPLYQRIGWPLYRKYGHAFEAFKLIVADPDA-ILDSLTYEEKETGADGQEVTKVVPA 179

Query: 181 VTPEIKDALIKNIRRRMTPQPHKIRADIDMKCFQYDGVLHIQEAMRKAEAAGNKDCPVKI 240
           VTPE+KDAL+KNIRRRMTPQP KIRAD++MKCFQ+DGVLHI++AMRKAEAAGN +CPVKI
Sbjct: 180 VTPEVKDALVKNIRRRMTPQPLKIRADVEMKCFQFDGVLHIKQAMRKAEAAGNDNCPVKI 239

Query: 241 KLVAAPLYVLTTETLDKHQGISVLNNAIKACGETIEKHKGKLVVKEAPRAVSEREDRLFM 300
           KLVA PLYVLTT+TLDK QGISVL +AIKAC   IEKHKGKL+VKEAPRAVSERED+LF 
Sbjct: 240 KLVAPPLYVLTTQTLDKDQGISVLTDAIKACTAEIEKHKGKLIVKEAPRAVSEREDKLFN 299

Query: 301 DDIEKLKIANXXX--XXXXXXXXXXXXXXVDLTKTGVGS 337
           + IE L   N                   +DLT +GV +
Sbjct: 300 EHIETLNEQNAEVDGDADSEEEEDTGMGDIDLTNSGVNA 338



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,891,850
Number of extensions: 375974
Number of successful extensions: 756
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 750
Number of HSP's successfully gapped: 2
Length of query: 338
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 237
Effective length of database: 11,762,187
Effective search space: 2787638319
Effective search space used: 2787638319
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)