BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os07g0676200 Os07g0676200|AK100834
(74 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os07g0676200 Similar to Ferredoxin-dependent glutamate synt... 155 5e-39
Os07g0658400 Similar to Ferredoxin-dependent glutamate synt... 93 4e-20
AK110476 73 4e-14
>Os07g0676200 Similar to Ferredoxin-dependent glutamate synthase, chloroplast
precursor (EC 1.4.7.1) (Fd-GOGAT)
Length = 74
Score = 155 bits (393), Expect = 5e-39, Method: Compositional matrix adjust.
Identities = 74/74 (100%), Positives = 74/74 (100%)
Query: 1 ADYDGGTGARPISLIKHSAGPWELGLSEAHQDGRVDGGFRSCQYVLMASVMGVDEYGFGS 60
ADYDGGTGARPISLIKHSAGPWELGLSEAHQDGRVDGGFRSCQYVLMASVMGVDEYGFGS
Sbjct: 1 ADYDGGTGARPISLIKHSAGPWELGLSEAHQDGRVDGGFRSCQYVLMASVMGVDEYGFGS 60
Query: 61 VAMNSYWMCSRCPH 74
VAMNSYWMCSRCPH
Sbjct: 61 VAMNSYWMCSRCPH 74
>Os07g0658400 Similar to Ferredoxin-dependent glutamate synthase, chloroplast
precursor (EC 1.4.7.1) (Fd-GOGAT)
Length = 1166
Score = 93.2 bits (230), Expect = 4e-20, Method: Composition-based stats.
Identities = 48/72 (66%), Positives = 52/72 (72%), Gaps = 11/72 (15%)
Query: 3 YDGGTGARPISLIKHSAGPWELGLSEAH----QDG-------RVDGGFRSCQYVLMASVM 51
+DGGTGA PIS IKH+ GPWELGLSE H Q+G RVDGGFRS VLMA+ M
Sbjct: 703 HDGGTGASPISSIKHAGGPWELGLSETHQTLIQNGLRERVVLRVDGGFRSGLDVLMAAAM 762
Query: 52 GVDEYGFGSVAM 63
G DEYGFGSVAM
Sbjct: 763 GADEYGFGSVAM 774
>AK110476
Length = 561
Score = 73.2 bits (178), Expect = 4e-14, Method: Composition-based stats.
Identities = 38/72 (52%), Positives = 48/72 (66%), Gaps = 11/72 (15%)
Query: 3 YDGGTGARPISLIKHSAGPWELGLSEAHQ-----------DGRVDGGFRSCQYVLMASVM 51
+DGGTGA PIS IKH+ GP E+G++E HQ R DGGFRS + VL A+ +
Sbjct: 96 HDGGTGASPISSIKHAGGPVEMGVAETHQVLVRNELRERVVLRADGGFRSGRDVLAAAAL 155
Query: 52 GVDEYGFGSVAM 63
G DE+GFG+VAM
Sbjct: 156 GADEFGFGTVAM 167
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.320 0.137 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,128,266
Number of extensions: 118239
Number of successful extensions: 178
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 177
Number of HSP's successfully gapped: 3
Length of query: 74
Length of database: 17,035,801
Length adjustment: 45
Effective length of query: 29
Effective length of database: 14,686,171
Effective search space: 425898959
Effective search space used: 425898959
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)