BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0587100 Os07g0587100|J100068C02
         (426 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0587100  Reticulon family protein                            797   0.0  
Os05g0513700  Reticulon family protein                             73   5e-13

>Os07g0587100 Reticulon family protein
          Length = 426

 Score =  797 bits (2059), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 391/426 (91%), Positives = 391/426 (91%)

Query: 1   MASRREXXXXXXXXXXXXXXXXXXXXRIXXXXXXXXXXXXXXXFLYPDGLLSRSSDAALH 60
           MASRRE                    RI               FLYPDGLLSRSSDAALH
Sbjct: 1   MASRREGPLMRGGAGGGAGQPLSRGSRIAAAVAVGVALGCVCAFLYPDGLLSRSSDAALH 60

Query: 61  WPRQADSVACETSEGVTSLKSHVVLLERKNAEFRKQINELTMKLQLAGQGKDEVLYKAGP 120
           WPRQADSVACETSEGVTSLKSHVVLLERKNAEFRKQINELTMKLQLAGQGKDEVLYKAGP
Sbjct: 61  WPRQADSVACETSEGVTSLKSHVVLLERKNAEFRKQINELTMKLQLAGQGKDEVLYKAGP 120

Query: 121 FGTVKAIRKNPTVIPDESINPRLAKILQQVAIKKELIVALANSNVREMLEMWFTNIKRVG 180
           FGTVKAIRKNPTVIPDESINPRLAKILQQVAIKKELIVALANSNVREMLEMWFTNIKRVG
Sbjct: 121 FGTVKAIRKNPTVIPDESINPRLAKILQQVAIKKELIVALANSNVREMLEMWFTNIKRVG 180

Query: 181 ISNYLIVALDDSTETFCKSKGVPFYRRDPDEGVDNIGKVGGNHAVSGLKFRILREFLQLG 240
           ISNYLIVALDDSTETFCKSKGVPFYRRDPDEGVDNIGKVGGNHAVSGLKFRILREFLQLG
Sbjct: 181 ISNYLIVALDDSTETFCKSKGVPFYRRDPDEGVDNIGKVGGNHAVSGLKFRILREFLQLG 240

Query: 241 YSVLLSDIDIIFFQNPFDHLYRDADVESMSDGHDNRTAYGFNDVFDEPLMGWARYAHTMR 300
           YSVLLSDIDIIFFQNPFDHLYRDADVESMSDGHDNRTAYGFNDVFDEPLMGWARYAHTMR
Sbjct: 241 YSVLLSDIDIIFFQNPFDHLYRDADVESMSDGHDNRTAYGFNDVFDEPLMGWARYAHTMR 300

Query: 301 IWVYNSGFFFIRPTIPSIELLDRVAGRLSREPKSWDQAVFNEELFFPSHPGYEGLHISKR 360
           IWVYNSGFFFIRPTIPSIELLDRVAGRLSREPKSWDQAVFNEELFFPSHPGYEGLHISKR
Sbjct: 301 IWVYNSGFFFIRPTIPSIELLDRVAGRLSREPKSWDQAVFNEELFFPSHPGYEGLHISKR 360

Query: 361 TMDIYQFMNSKVLFKTVRKDANLRKLKPVIVHLNYHPDKSERMKAVIEFYVNGKQNALEH 420
           TMDIYQFMNSKVLFKTVRKDANLRKLKPVIVHLNYHPDKSERMKAVIEFYVNGKQNALEH
Sbjct: 361 TMDIYQFMNSKVLFKTVRKDANLRKLKPVIVHLNYHPDKSERMKAVIEFYVNGKQNALEH 420

Query: 421 FPDGSE 426
           FPDGSE
Sbjct: 421 FPDGSE 426



>Os05g0513700 Reticulon family protein
          Length = 689

 Score = 72.8 bits (177), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 61/206 (29%), Positives = 107/206 (51%), Gaps = 31/206 (15%)

Query: 143 LAKILQQVAIK-KELIVALANSNVREMLEMWFTNIKRVGISNYLIVALDDSTETFCKSKG 201
           L  +L+ VA K + +++A+A ++ R+ML  W   ++R+ ++N+++ ALD  T  F   +G
Sbjct: 391 LEMLLELVADKNRSVVLAVAGASYRDMLMSWVCRLRRLRVTNFVVCALDQETYEFSVLQG 450

Query: 202 VPFYRR--DPDEGVDNIGKVGGN--HAVSGLKFRILREFLQLGYSVLLSDIDIIFFQNPF 257
           +P  R    P+    +    G      V+ +K RI+ + L+LGY+VLLSD+D+ +F NP 
Sbjct: 451 MPVSRDTLSPNNVSFDDCHFGTQCFQQVTKVKSRIVLKILRLGYNVLLSDVDVYWFHNPV 510

Query: 258 DHLYRDADVESMSDGHDNRTAYGFNDVFDE--PLMGWARYAHTMRIWVYNSGFFFIR--- 312
             L+      S+  G    T    +D F++  P+    R          NSGF++ R   
Sbjct: 511 SFLH------SLGPG----TFAAQSDEFNQTGPINMPRR---------LNSGFYYARSDD 551

Query: 313 PTIPSIELLDRVAGR--LSREPKSWD 336
            TI ++E++ + A    LS +P  +D
Sbjct: 552 ATITAMEMIVKHATNSGLSEQPSFYD 577



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.138    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,863,580
Number of extensions: 530257
Number of successful extensions: 1225
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1227
Number of HSP's successfully gapped: 2
Length of query: 426
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 322
Effective length of database: 11,605,545
Effective search space: 3736985490
Effective search space used: 3736985490
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 157 (65.1 bits)