BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0136100 Os07g0136100|Os07g0136100
         (95 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0136100  Conserved hypothetical protein                      153   3e-38
Os07g0135500                                                      113   2e-26

>Os07g0136100 Conserved hypothetical protein
          Length = 95

 Score =  153 bits (386), Expect = 3e-38,   Method: Compositional matrix adjust.
 Identities = 79/95 (83%), Positives = 79/95 (83%)

Query: 1  MAAMAMRTLARKVMPVXXXXXXXXXXXXXXXXHRLEGTASPKTTTPTGKGRASTLEEDIR 60
          MAAMAMRTLARKVMPV                HRLEGTASPKTTTPTGKGRASTLEEDIR
Sbjct: 1  MAAMAMRTLARKVMPVPAALRRAPPPPPSRSLHRLEGTASPKTTTPTGKGRASTLEEDIR 60

Query: 61 FLAELAKRHADEARYYRIMALSAVSASGAVFFTLC 95
          FLAELAKRHADEARYYRIMALSAVSASGAVFFTLC
Sbjct: 61 FLAELAKRHADEARYYRIMALSAVSASGAVFFTLC 95



>Os07g0135500 
          Length = 77

 Score =  113 bits (283), Expect = 2e-26,   Method: Compositional matrix adjust.
 Identities = 57/63 (90%), Positives = 59/63 (93%), Gaps = 1/63 (1%)

Query: 33 HRLEGTASPKTTTPTGKGRASTLEED-IRFLAELAKRHADEARYYRIMALSAVSASGAVF 91
          H+  GTASPKTTTPTGKGRASTLEED IRFLAELAKRHADEARYYRI+ALSAVSASG VF
Sbjct: 2  HQFRGTASPKTTTPTGKGRASTLEEDNIRFLAELAKRHADEARYYRIVALSAVSASGVVF 61

Query: 92 FTL 94
          FTL
Sbjct: 62 FTL 64



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.128    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,225,218
Number of extensions: 65687
Number of successful extensions: 339
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 336
Number of HSP's successfully gapped: 3
Length of query: 95
Length of database: 17,035,801
Length adjustment: 64
Effective length of query: 31
Effective length of database: 13,694,105
Effective search space: 424517255
Effective search space used: 424517255
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 149 (62.0 bits)