BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0711800 Os06g0711800|AK069642
         (200 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0711800  Pectinesterase inhibitor domain containing pro...   277   3e-75
Os08g0330400  Pectinesterase inhibitor domain containing pro...   202   1e-52
Os04g0106000  Pectinesterase inhibitor domain containing pro...   112   1e-25
Os10g0185400                                                       66   2e-11

>Os06g0711800 Pectinesterase inhibitor domain containing protein
          Length = 200

 Score =  277 bits (709), Expect = 3e-75,   Method: Compositional matrix adjust.
 Identities = 140/170 (82%), Positives = 140/170 (82%)

Query: 31  DFIRKSCRATQYPAVCVQXXXXXXXXXXXXXXXXRELARAALSVSVDKARSASTYVGHIC 90
           DFIRKSCRATQYPAVCVQ                RELARAALSVSVDKARSASTYVGHIC
Sbjct: 31  DFIRKSCRATQYPAVCVQSLASYGGAGAPPPRSPRELARAALSVSVDKARSASTYVGHIC 90

Query: 91  XXXXXXXXXXXXXXVRDCLENMADSVGHLRDAAQELGGNMGRAGSPGFKWHLSNVQTWCS 150
                         VRDCLENMADSVGHLRDAAQELGGNMGRAGSPGFKWHLSNVQTWCS
Sbjct: 91  GPGGGRGGAGGAGPVRDCLENMADSVGHLRDAAQELGGNMGRAGSPGFKWHLSNVQTWCS 150

Query: 151 AALTDENTCLDGLSRGVDAATRSAIRGKIVEVAQVTSNALALVNRVAPAN 200
           AALTDENTCLDGLSRGVDAATRSAIRGKIVEVAQVTSNALALVNRVAPAN
Sbjct: 151 AALTDENTCLDGLSRGVDAATRSAIRGKIVEVAQVTSNALALVNRVAPAN 200



>Os08g0330400 Pectinesterase inhibitor domain containing protein
          Length = 202

 Score =  202 bits (514), Expect = 1e-52,   Method: Compositional matrix adjust.
 Identities = 107/150 (71%), Positives = 110/150 (73%), Gaps = 5/150 (3%)

Query: 31  DFIRKSCRATQYPAVCVQXXXXXXXXXXXXXXXXRELARAALSVSVDKARSASTYVGHIC 90
           +FIRK CRATQYPAVCVQ                 EL  A LSVSVDKA+S S YVG IC
Sbjct: 25  NFIRKLCRATQYPAVCVQSQASYGGAPLPRSPP--ELVHAVLSVSVDKAQSVSMYVGRIC 82

Query: 91  XXXXXXXXXXXXXXVRDCLENMADSVGHLRDAAQELGGNMGRAGSPGFKWHLSNVQTWCS 150
                         VRDCLENMADSVGHL DAAQELGGNMGRAGSPGFKWHLSNVQTWCS
Sbjct: 83  GPGGSRGVAGP---VRDCLENMADSVGHLCDAAQELGGNMGRAGSPGFKWHLSNVQTWCS 139

Query: 151 AALTDENTCLDGLSRGVDAATRSAIRGKIV 180
           AALTD+NTCLDGLSRGVDAATRSA RGKIV
Sbjct: 140 AALTDKNTCLDGLSRGVDAATRSATRGKIV 169



>Os04g0106000 Pectinesterase inhibitor domain containing protein
          Length = 177

 Score =  112 bits (281), Expect = 1e-25,   Method: Compositional matrix adjust.
 Identities = 76/174 (43%), Positives = 98/174 (56%), Gaps = 13/174 (7%)

Query: 31  DFIRKSCRATQYPAVCVQXXXXXXXXXXXXXXXXRELARAALSVSVDKARSASTYVGHIC 90
           DF+R+SCRAT+YP VC +                R LA+AAL+V  D+ARS S Y+G   
Sbjct: 7   DFVRRSCRATRYPQVCERSLMPQAPAVGRSP---RLLAQAALTVGADRARSCSGYLGGGG 63

Query: 91  XXXXXXXXXXXXX--XVRDCLENMADSVGHLRDAAQELGGNMGRAGSPGFKWHLSNVQTW 148
                           V DC + + D+   LR +A E+   MGR+GSP F W LSNVQTW
Sbjct: 64  SSSSSKRSGGGGRGGAVGDCADTLRDAEERLRQSAAEMS-RMGRSGSPRFAWRLSNVQTW 122

Query: 149 CSAALTDENTCLDGLSR----GVDAATRSAIRGKIVEVAQVTSNALALVNRVAP 198
            SAALTD +TCLD L+     G+D     A+R ++  V+Q TSNALALVN + P
Sbjct: 123 ASAALTDASTCLDSLATYAAPGIDV---DALRKRVGAVSQATSNALALVNNLDP 173



>Os10g0185400 
          Length = 212

 Score = 66.2 bits (160), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 55/174 (31%), Positives = 77/174 (44%), Gaps = 15/174 (8%)

Query: 32  FIRKSCRATQYPAVCVQXXXXXXXXXXXXXXXXRELARAALSVSVDKARSASTYVGHICX 91
           F+R  C  T+YP VC                   +LA AA +V+  K R+ S  +  +  
Sbjct: 43  FLRSRCATTRYPDVCYDSLLPYASTFQTSHV---KLAVAAANVAAAKLRAFSARINDLLA 99

Query: 92  XXXXXXXXXXXXXVRDCLENMADSVGHLRDAAQELG-----GNMGRAGSPGFKWHLSNVQ 146
                        ++DC   ++D+    R ++ ELG            S   +WH+SNVQ
Sbjct: 100 QGGAARVDAA---LKDCKSTISDAGDLARQSSAELGQLDAGAAAAGVSSRQARWHVSNVQ 156

Query: 147 TWCSAALTDENTCLDGLSRGVDAATRSA----IRGKIVEVAQVTSNALALVNRV 196
           TW SAA+TDE TC DG     +AA  S     +   +  V Q TS ALALVN +
Sbjct: 157 TWLSAAITDEGTCTDGFEEAGEAAAGSPAGKEVAAGVARVKQHTSIALALVNGI 210



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.129    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,206,190
Number of extensions: 132187
Number of successful extensions: 346
Number of sequences better than 1.0e-10: 7
Number of HSP's gapped: 335
Number of HSP's successfully gapped: 7
Length of query: 200
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 104
Effective length of database: 12,023,257
Effective search space: 1250418728
Effective search space used: 1250418728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 153 (63.5 bits)