BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0650900 Os06g0650900|AK107961
         (142 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0650900  Heat shock protein DnaJ family protein              225   7e-60

>Os06g0650900 Heat shock protein DnaJ family protein
          Length = 142

 Score =  225 bits (574), Expect = 7e-60,   Method: Compositional matrix adjust.
 Identities = 110/142 (77%), Positives = 110/142 (77%)

Query: 1   MAAGGMLCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGPGGPVEDHYRTLRLPPGAT 60
           MAAGGMLC                                GPGGPVEDHYRTLRLPPGAT
Sbjct: 1   MAAGGMLCVSSSPTASASVARGRRQRRRSVEVRCSSVAAAGPGGPVEDHYRTLRLPPGAT 60

Query: 61  KGEVKRAFRRLALTYHPDVSKESDSGVHFQRINVAYQMVMGNMREAEERLEYWRLKYGLD 120
           KGEVKRAFRRLALTYHPDVSKESDSGVHFQRINVAYQMVMGNMREAEERLEYWRLKYGLD
Sbjct: 61  KGEVKRAFRRLALTYHPDVSKESDSGVHFQRINVAYQMVMGNMREAEERLEYWRLKYGLD 120

Query: 121 DEDLDKYRNHLNDEDDDEWFDV 142
           DEDLDKYRNHLNDEDDDEWFDV
Sbjct: 121 DEDLDKYRNHLNDEDDDEWFDV 142



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.139    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,614,668
Number of extensions: 169401
Number of successful extensions: 755
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 756
Number of HSP's successfully gapped: 1
Length of query: 142
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 51
Effective length of database: 12,284,327
Effective search space: 626500677
Effective search space used: 626500677
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)