BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0354500 Os06g0354500|AK066934
         (685 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0354500  Similar to Acyl-coenzyme A oxidase 3, peroxiso...  1335   0.0  
Os06g0103500  Similar to Acyl-coenzyme A oxidase 1, peroxiso...   250   3e-66

>Os06g0354500 Similar to Acyl-coenzyme A oxidase 3, peroxisomal precursor (EC
           1.3.3.6) (AOX 3) (Medium-chain acyl-CoA oxidase) (AtCX3)
          Length = 685

 Score = 1335 bits (3456), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 648/685 (94%), Positives = 648/685 (94%)

Query: 1   MDPSYPPSXXXXXXXXXXXXXXGLSPRDXXXXXXXLEPSACLSYXXXXXXXXXXXXXXXX 60
           MDPSYPPS              GLSPRD       LEPSACLSY                
Sbjct: 1   MDPSYPPSATARRAAAIARHLAGLSPRDAAAVAAALEPSACLSYAPPESSEPAPAFSPLE 60

Query: 61  LRSLLDGHHLRERDWAFRAMEESPLFCQRRSGGKVFVSPDYNEGKEGQREATMRRVGYLA 120
           LRSLLDGHHLRERDWAFRAMEESPLFCQRRSGGKVFVSPDYNEGKEGQREATMRRVGYLA
Sbjct: 61  LRSLLDGHHLRERDWAFRAMEESPLFCQRRSGGKVFVSPDYNEGKEGQREATMRRVGYLA 120

Query: 121 RRGVFRGWLTEPGPDAELRKLALLECLGMYDHSLAIKIGVHFFLWGSAIKFLGTKRHHDK 180
           RRGVFRGWLTEPGPDAELRKLALLECLGMYDHSLAIKIGVHFFLWGSAIKFLGTKRHHDK
Sbjct: 121 RRGVFRGWLTEPGPDAELRKLALLECLGMYDHSLAIKIGVHFFLWGSAIKFLGTKRHHDK 180

Query: 181 WLSDTENYVIKGCFSMTELGHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAAN 240
           WLSDTENYVIKGCFSMTELGHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAAN
Sbjct: 181 WLSDTENYVIKGCFSMTELGHGSNVRGIETVATYDIKTREFVINTPCESAQKYWIGGAAN 240

Query: 241 HATHTIVFAQLHINGRNEGVHAFVAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFN 300
           HATHTIVFAQLHINGRNEGVHAFVAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFN
Sbjct: 241 HATHTIVFAQLHINGRNEGVHAFVAQIRDEHENVMPNIQIADCGHKIGLNGVDNGRIWFN 300

Query: 301 NIRVPRENLLNLVADVLPDGQYVSTIDDPDQRFAAFLSPLTLGRVNIAVNAVYISKVGVA 360
           NIRVPRENLLNLVADVLPDGQYVSTIDDPDQRFAAFLSPLTLGRVNIAVNAVYISKVGVA
Sbjct: 301 NIRVPRENLLNLVADVLPDGQYVSTIDDPDQRFAAFLSPLTLGRVNIAVNAVYISKVGVA 360

Query: 361 IAVRYALSRRAFSVTPDGPEMLLLDYPSHQRRLLPLLAKACLMSSAGNFMKRMYVKRTPE 420
           IAVRYALSRRAFSVTPDGPEMLLLDYPSHQRRLLPLLAKACLMSSAGNFMKRMYVKRTPE
Sbjct: 361 IAVRYALSRRAFSVTPDGPEMLLLDYPSHQRRLLPLLAKACLMSSAGNFMKRMYVKRTPE 420

Query: 421 LNKSIHIYSSALKATLTWQNMTTLQECREACGGQGLKTENRIGIFKAEFDVQSTFEGDNN 480
           LNKSIHIYSSALKATLTWQNMTTLQECREACGGQGLKTENRIGIFKAEFDVQSTFEGDNN
Sbjct: 421 LNKSIHIYSSALKATLTWQNMTTLQECREACGGQGLKTENRIGIFKAEFDVQSTFEGDNN 480

Query: 481 VLMQQVSKALYAEFLTAKRKNQPFKGLGLEHLNGPCPVIPDYLTSGTLRSSSFQMDLLCL 540
           VLMQQVSKALYAEFLTAKRKNQPFKGLGLEHLNGPCPVIPDYLTSGTLRSSSFQMDLLCL
Sbjct: 481 VLMQQVSKALYAEFLTAKRKNQPFKGLGLEHLNGPCPVIPDYLTSGTLRSSSFQMDLLCL 540

Query: 541 RERDLLKRFTTEVSNYLAQGENREKALMLSYQLAEDLARAFTERTILQIFLEDEKNIPTG 600
           RERDLLKRFTTEVSNYLAQGENREKALMLSYQLAEDLARAFTERTILQIFLEDEKNIPTG
Sbjct: 541 RERDLLKRFTTEVSNYLAQGENREKALMLSYQLAEDLARAFTERTILQIFLEDEKNIPTG 600

Query: 601 SLKDILGLLRSLYVMVCIDESASFLRYGCLSRENVAAARKEVMTLCSELRPHALAIVSSF 660
           SLKDILGLLRSLYVMVCIDESASFLRYGCLSRENVAAARKEVMTLCSELRPHALAIVSSF
Sbjct: 601 SLKDILGLLRSLYVMVCIDESASFLRYGCLSRENVAAARKEVMTLCSELRPHALAIVSSF 660

Query: 661 GIPDAFLSPLAFDWIEANARSSGNE 685
           GIPDAFLSPLAFDWIEANARSSGNE
Sbjct: 661 GIPDAFLSPLAFDWIEANARSSGNE 685



>Os06g0103500 Similar to Acyl-coenzyme A oxidase 1, peroxisomal (EC 1.3.3.6) (AOX
           1) (Long- chain acyl-CoA oxidase) (AtCX1)
          Length = 669

 Score =  250 bits (638), Expect = 3e-66,   Method: Compositional matrix adjust.
 Identities = 164/536 (30%), Positives = 270/536 (50%), Gaps = 40/536 (7%)

Query: 158 IGVHFFLWGSAIKFLGTKRHHDKWLSDTENYVIKGCFSMTELGHGSNVRGIETVATYDIK 217
           + +H+ ++  AIK  GT+    KWL     + I GC++ TELGHGSNV+G+ET AT+D K
Sbjct: 104 VDLHWGMFVPAIKGQGTEEQQKKWLPLAYRFQIIGCYAQTELGHGSNVQGLETTATFDPK 163

Query: 218 TREFVINTPCESAQKYWIGGAANHATHTIVFAQLHINGRNEGVHAFVAQIRD-EHENVMP 276
           T EFVI++P  ++ K+W GG    +TH +V+A+L   G++ G+H F+ Q+R  E  + +P
Sbjct: 164 TDEFVIHSPTLTSSKWWPGGLGKASTHAVVYARLITEGKDYGIHGFIVQLRSLEDHSPLP 223

Query: 277 NIQIADCGHKIG---LNGVDNGRIWFNNIRVPRENLLNLVADVLPDGQYVSTIDDPDQRF 333
            + + D G K G    N +DNG + F+++R+PR+ +L  ++ V  +G+YV + D P Q  
Sbjct: 224 GVTLGDIGGKFGSGAYNSMDNGVLRFDHVRIPRDQMLMRLSQVTKEGKYVHS-DVPKQ-- 280

Query: 334 AAFLSPLTLGRVNIAVNAVYISKVGVAIAVRYALSRRAFSVTPDGPEMLLLDYPSHQRRL 393
                 +   R  I  +A         IAVRY+  R+ F     GPE  +L+Y + Q RL
Sbjct: 281 -LLYGTMVFVRQTIVADASKALSRATCIAVRYSAIRKQFGPQTGGPETQVLNYKTQQSRL 339

Query: 394 LPLLAKACLMSSAGNFMKRMYVKRTPELN-------KSIHIYSSALKATLTWQNMTTLQE 446
            PLLA A      G ++K +Y   T +L        +  H  ++ LKA  T      ++E
Sbjct: 340 FPLLASAYAFRFVGEWLKWLYTDVTHKLEAKDFSTLQEAHACTAGLKAVTTSATADGIEE 399

Query: 447 CREACGGQGLKTENRIGIFKAEFDVQSTFEGDNNVLMQQVSKALYAEFLTAKRKNQP--- 503
           CR+ CGG G    + +    A +    T+EGDN VL+ QV++ L           QP   
Sbjct: 400 CRKLCGGHGYLNSSGLPELFAIYVPACTYEGDNVVLLLQVARFLMKTVSQLASGKQPVGT 459

Query: 504 --FKGLGLEHLNGPCPV--IPDYLTSGTLRSSSFQMDLLCLRERDLLKRFTTEVSNYLAQ 559
             + G     +   C V    D+L    +R             R L  R     +  + +
Sbjct: 460 TAYMGNIQYLMQCKCGVNTAEDWLNPAAIRE--------VFEARAL--RMAVNCAQNINK 509

Query: 560 GENREKALMLSYQLAEDL---ARAFTERTILQIFLED-EKNIPTGSLKDILGLLRSLYVM 615
             ++E+     Y+L+ DL   A A  +  I+  F+E  E++IP   +K+ L +L ++Y +
Sbjct: 510 APSQEEGF---YELSPDLLEVAVAHIQLIIVTKFIEKLEQDIPGEGVKEQLCILCNVYAL 566

Query: 616 VCIDES-ASFLRYGCLSRENVAAARKEVMTLCSELRPHALAIVSSFGIPDAFLSPL 670
             + +    FL  G ++    A A +++  L +++RP+A+A+V +F   D +L  +
Sbjct: 567 YLVHKHLGDFLSTGSITARQGALANEQLGKLYAQVRPNAVALVDAFNYTDHYLGSV 622



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 22,120,902
Number of extensions: 895999
Number of successful extensions: 1660
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1658
Number of HSP's successfully gapped: 2
Length of query: 685
Length of database: 17,035,801
Length adjustment: 108
Effective length of query: 577
Effective length of database: 11,396,689
Effective search space: 6575889553
Effective search space used: 6575889553
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 159 (65.9 bits)