BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0543200 Os05g0543200|J023134L19
         (544 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0543200  Similar to Ran GTPase activating protein           1037   0.0  
Os05g0409200                                                      194   2e-49

>Os05g0543200 Similar to Ran GTPase activating protein
          Length = 544

 Score = 1037 bits (2681), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 518/544 (95%), Positives = 518/544 (95%)

Query: 1   MDSTKQDFQPRTFSIKLWPPSESTRLMLVERMTKNLSTESIFSRKYGLLGKEEAHDNAKR 60
           MDSTKQDFQPRTFSIKLWPPSESTRLMLVERMTKNLSTESIFSRKYGLLGKEEAHDNAKR
Sbjct: 1   MDSTKQDFQPRTFSIKLWPPSESTRLMLVERMTKNLSTESIFSRKYGLLGKEEAHDNAKR 60

Query: 61  IEEVCFASADEHFKEEPDGDGSSAVQLYAKETSKLMLEVLKRGPRTTVEPEVPVADTPLE 120
           IEEVCFASADEHFKEEPDGDGSSAVQLYAKETSKLMLEVLKRGPRTTVEPEVPVADTPLE
Sbjct: 61  IEEVCFASADEHFKEEPDGDGSSAVQLYAKETSKLMLEVLKRGPRTTVEPEVPVADTPLE 120

Query: 121 PADSVFDISGGKRAFIEADEAKELLSPLIKPGNAYKRICFSNRSFGIGAANVAGPILESI 180
           PADSVFDISGGKRAFIEADEAKELLSPLIKPGNAYKRICFSNRSFGIGAANVAGPILESI
Sbjct: 121 PADSVFDISGGKRAFIEADEAKELLSPLIKPGNAYKRICFSNRSFGIGAANVAGPILESI 180

Query: 181 KKQLTEVDISDFVAGRPEDEALDVMRIFSKALEGAVLRYLNISDNALGEKGVRAFEELLK 240
           KKQLTEVDISDFVAGRPEDEALDVMRIFSKALEGAVLRYLNISDNALGEKGVRAFEELLK
Sbjct: 181 KKQLTEVDISDFVAGRPEDEALDVMRIFSKALEGAVLRYLNISDNALGEKGVRAFEELLK 240

Query: 241 SQDNLEELYVMNDGISEEAAQALSELIPSTEKLKILHFHNNMTGDEGAMFIAEMVKRSPN 300
           SQDNLEELYVMNDGISEEAAQALSELIPSTEKLKILHFHNNMTGDEGAMFIAEMVKRSPN
Sbjct: 241 SQDNLEELYVMNDGISEEAAQALSELIPSTEKLKILHFHNNMTGDEGAMFIAEMVKRSPN 300

Query: 301 LESFRCSATRIGSDGGVALAEALGTCTRLKKLDLRDNLFGVEAGLALSKTLSKLPDLVEL 360
           LESFRCSATRIGSDGGVALAEALGTCTRLKKLDLRDNLFGVEAGLALSKTLSKLPDLVEL
Sbjct: 301 LESFRCSATRIGSDGGVALAEALGTCTRLKKLDLRDNLFGVEAGLALSKTLSKLPDLVEL 360

Query: 361 YLSDLNLENKGTVAIINTLKQSAPQLEVLEMAGNEINAKASQALAECLTAMQSLKKLTLA 420
           YLSDLNLENKGTVAIINTLKQSAPQLEVLEMAGNEINAKASQALAECLTAMQSLKKLTLA
Sbjct: 361 YLSDLNLENKGTVAIINTLKQSAPQLEVLEMAGNEINAKASQALAECLTAMQSLKKLTLA 420

Query: 421 ENELKDDGAVVIAKSLEDGHQDLKELDVSTNMLQRVGARCFAQAIANKPGFVQLNINGNF 480
           ENELKDDGAVVIAKSLEDGHQDLKELDVSTNMLQRVGARCFAQAIANKPGFVQLNINGNF
Sbjct: 421 ENELKDDGAVVIAKSLEDGHQDLKELDVSTNMLQRVGARCFAQAIANKPGFVQLNINGNF 480

Query: 481 ISDEGIDEVKDILKSGENSVEVLGPLXXXXXXXXXXXXXXXXXXXXXXXXXXSKLQSLKV 540
           ISDEGIDEVKDILKSGENSVEVLGPL                          SKLQSLKV
Sbjct: 481 ISDEGIDEVKDILKSGENSVEVLGPLDENDPEGEAEDDEEEEEEEEDDGELDSKLQSLKV 540

Query: 541 EQDD 544
           EQDD
Sbjct: 541 EQDD 544



>Os05g0409200 
          Length = 227

 Score =  194 bits (492), Expect = 2e-49,   Method: Compositional matrix adjust.
 Identities = 124/258 (48%), Positives = 147/258 (56%), Gaps = 63/258 (24%)

Query: 201 ALDVMRIFSKALEGAVLRYLNISDNALGEKGVRAFEELLKSQDNLEELYVMNDGISEEAA 260
           A DVMRI SK LEG VLRYLNISDNA GEKGVRAF          EEL    D + EE  
Sbjct: 2   AFDVMRISSKVLEGDVLRYLNISDNAYGEKGVRAF----------EELLKSQDTLEEEE- 50

Query: 261 QALSELIPSTEKLKILHFHNNMTGDEGAMFIAEMVKRSPNLESFRCSATRIGSDGGVALA 320
                          L+  NN   +E A    E++   P+ E                  
Sbjct: 51  ---------------LYMMNNSISEEAAKTFYELI---PSTE------------------ 74

Query: 321 EALGTCTRLKKLDLRDNLFGVEAGLALSKTLSKLPDLVELYLSDLNLENKGTVAIINTLK 380
                  +LK L   +N+ G E  + +++ +          L DLNLENKGT+AI+NT K
Sbjct: 75  -------KLKVLHFHNNMMGDEGAMFVAEMI---------LLGDLNLENKGTLAIVNTRK 118

Query: 381 QSAPQLEVLEMAGNEINAKASQALAECLTAMQSLKKLTLAENELKDDGAVVIAKSLEDGH 440
           Q  PQ+EVLEMA N+INAK +QALAECLT +QSLKKLTLAEN LKDDGAVVIAK+LEDGH
Sbjct: 119 QLTPQIEVLEMARNKINAKEAQALAECLTTLQSLKKLTLAENGLKDDGAVVIAKALEDGH 178

Query: 441 QDLKELDVSTNMLQRVGA 458
           QDLKELDVS   +   G+
Sbjct: 179 QDLKELDVSKEYVAEDGS 196



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.132    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,852,091
Number of extensions: 634926
Number of successful extensions: 1813
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1799
Number of HSP's successfully gapped: 3
Length of query: 544
Length of database: 17,035,801
Length adjustment: 106
Effective length of query: 438
Effective length of database: 11,501,117
Effective search space: 5037489246
Effective search space used: 5037489246
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 158 (65.5 bits)