BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0494200 Os05g0494200|J05595
         (108 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0494200  Similar to Cysteine proteinase inhibitor-II (O...   194   2e-50
Os01g0803200  Cysteine proteinase inhibitor-I (Oryzacystatin-I)   100   2e-22
AK105278                                                          100   2e-22
Os01g0270100  Similar to Cysteine protease inhibitor               79   5e-16

>Os05g0494200 Similar to Cysteine proteinase inhibitor-II (Oryzacystatin-II)
          Length = 108

 Score =  194 bits (492), Expect = 2e-50,   Method: Compositional matrix adjust.
 Identities = 96/108 (88%), Positives = 96/108 (88%)

Query: 1   MAEEAQQPRGVKVGGIHDAPAGRENDLTTVELARFAVAEHNSKANAMLELEXXXXXXXXX 60
           MAEEAQQPRGVKVGGIHDAPAGRENDLTTVELARFAVAEHNSKANAMLELE         
Sbjct: 1   MAEEAQQPRGVKVGGIHDAPAGRENDLTTVELARFAVAEHNSKANAMLELERVVKVRQQV 60

Query: 61  XXXFMHYLTVEVKEPGGANKLYEAKVWERAWENFKQLQDFKPLDDATA 108
              FMHYLTVEVKEPGGANKLYEAKVWERAWENFKQLQDFKPLDDATA
Sbjct: 61  VGGFMHYLTVEVKEPGGANKLYEAKVWERAWENFKQLQDFKPLDDATA 108



>Os01g0803200 Cysteine proteinase inhibitor-I (Oryzacystatin-I)
          Length = 140

 Score =  100 bits (250), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 51/95 (53%), Positives = 64/95 (67%), Gaps = 3/95 (3%)

Query: 10  GVKVGGIHDAPAGRENDLTTVELARFAVAEHNSKANAMLELEXXXXXXXXXXXXFMHYLT 69
           G  +GG+   P G ENDL  V+LARFAV EHN KAN++LE E             ++Y T
Sbjct: 44  GPVLGGVE--PVGNENDLHLVDLARFAVTEHNKKANSLLEFEKLVSVKQQVVAGTLYYFT 101

Query: 70  VEVKEPGGANKLYEAKVWERAWENFKQLQDFKPLD 104
           +EVKE G A KLYEAKVWE+ W +FK+LQ+FKP+D
Sbjct: 102 IEVKE-GDAKKLYEAKVWEKPWMDFKELQEFKPVD 135



>AK105278 
          Length = 102

 Score =  100 bits (250), Expect = 2e-22,   Method: Compositional matrix adjust.
 Identities = 51/95 (53%), Positives = 64/95 (67%), Gaps = 3/95 (3%)

Query: 10  GVKVGGIHDAPAGRENDLTTVELARFAVAEHNSKANAMLELEXXXXXXXXXXXXFMHYLT 69
           G  +GG+   P G ENDL  V+LARFAV EHN KAN++LE E             ++Y T
Sbjct: 6   GPVLGGVE--PVGNENDLHLVDLARFAVTEHNKKANSLLEFEKLVSVKQQVVAGTLYYFT 63

Query: 70  VEVKEPGGANKLYEAKVWERAWENFKQLQDFKPLD 104
           +EVKE G A KLYEAKVWE+ W +FK+LQ+FKP+D
Sbjct: 64  IEVKE-GDAKKLYEAKVWEKPWMDFKELQEFKPVD 97



>Os01g0270100 Similar to Cysteine protease inhibitor
          Length = 250

 Score = 79.3 bits (194), Expect = 5e-16,   Method: Compositional matrix adjust.
 Identities = 44/98 (44%), Positives = 58/98 (59%), Gaps = 2/98 (2%)

Query: 10  GVKVGGIHDAPAGRENDLTTVELARFAVAEHNSKANAMLELEXXXXXXXXXXXXFMHYLT 69
           G  +GG HDAP+   N + T  LARFAV EHN + NA+LE               +H+LT
Sbjct: 45  GHVLGGAHDAPSA-ANSVETDALARFAVDEHNKRENALLEFVRVVEAKEQVVAGTLHHLT 103

Query: 70  VEVKEPGGANKLYEAKVWERAWENFKQLQDFKPLDDAT 107
           +E  E  G  K+YEAKVW + W +FK+LQ+F+   DAT
Sbjct: 104 LEALE-AGRKKVYEAKVWVKPWLDFKELQEFRNTGDAT 140



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.129    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,338,281
Number of extensions: 92715
Number of successful extensions: 171
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 169
Number of HSP's successfully gapped: 4
Length of query: 108
Length of database: 17,035,801
Length adjustment: 76
Effective length of query: 32
Effective length of database: 13,067,537
Effective search space: 418161184
Effective search space used: 418161184
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)