BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0461000 Os05g0461000|AK066739
         (177 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0461000  Clathrin adaptor complex, small chain family p...   324   2e-89
Os01g0838800  Clathrin adaptor complex, small chain family p...   249   1e-66
Os02g0317400  Clathrin adaptor complex, small chain family p...   232   1e-61

>Os05g0461000 Clathrin adaptor complex, small chain family protein
          Length = 177

 Score =  324 bits (831), Expect = 2e-89,   Method: Compositional matrix adjust.
 Identities = 163/177 (92%), Positives = 163/177 (92%)

Query: 1   MESCPSVKNILLLDSEGKRVAVKYYTDDWPTLSAKLAFEKSVFVKTQKATAGAEAEIVMF 60
           MESCPSVKNILLLDSEGKRVAVKYYTDDWPTLSAKLAFEKSVFVKTQKATAGAEAEIVMF
Sbjct: 1   MESCPSVKNILLLDSEGKRVAVKYYTDDWPTLSAKLAFEKSVFVKTQKATAGAEAEIVMF 60

Query: 61  DGHIVVYKFIQDLHFFVTGGEEENELILASVLQGFTDAVDIILRNNVDKRTAXXXXXXXX 120
           DGHIVVYKFIQDLHFFVTGGEEENELILASVLQGFTDAVDIILRNNVDKRTA        
Sbjct: 61  DGHIVVYKFIQDLHFFVTGGEEENELILASVLQGFTDAVDIILRNNVDKRTALENLDLIL 120

Query: 121 XXXXXXVDGGIVLETEGSVIAEKVSAHGIEGATSLAEQTIVQALTTAREHLTKSLLM 177
                 VDGGIVLETEGSVIAEKVSAHGIEGATSLAEQTIVQALTTAREHLTKSLLM
Sbjct: 121 LCLDEIVDGGIVLETEGSVIAEKVSAHGIEGATSLAEQTIVQALTTAREHLTKSLLM 177



>Os01g0838800 Clathrin adaptor complex, small chain family protein
          Length = 177

 Score =  249 bits (635), Expect = 1e-66,   Method: Compositional matrix adjust.
 Identities = 119/176 (67%), Positives = 137/176 (77%)

Query: 1   MESCPSVKNILLLDSEGKRVAVKYYTDDWPTLSAKLAFEKSVFVKTQKATAGAEAEIVMF 60
           MESCPSVKNIL+LDSEGKRVAVKYY+DDWP+LS+K AFEKSVF KTQK +A  EAEIVMF
Sbjct: 1   MESCPSVKNILVLDSEGKRVAVKYYSDDWPSLSSKQAFEKSVFAKTQKTSARTEAEIVMF 60

Query: 61  DGHIVVYKFIQDLHFFVTGGEEENELILASVLQGFTDAVDIILRNNVDKRTAXXXXXXXX 120
           D + VVYKFIQDLHFFVTGG+EENELILASVLQGF++A+D +LRN V +R A        
Sbjct: 61  DSYFVVYKFIQDLHFFVTGGDEENELILASVLQGFSEAIDYLLRNKVHRRAALENLDLIF 120

Query: 121 XXXXXXVDGGIVLETEGSVIAEKVSAHGIEGATSLAEQTIVQALTTAREHLTKSLL 176
                 VDGGIVLET+   I EKVS HG+EG+ SL EQ +  AL TAREH  +S+ 
Sbjct: 121 LCLDEVVDGGIVLETDAKAILEKVSGHGLEGSGSLTEQKLSSALATAREHFARSIF 176



>Os02g0317400 Clathrin adaptor complex, small chain family protein
          Length = 182

 Score =  232 bits (591), Expect = 1e-61,   Method: Compositional matrix adjust.
 Identities = 110/175 (62%), Positives = 139/175 (79%)

Query: 2   ESCPSVKNILLLDSEGKRVAVKYYTDDWPTLSAKLAFEKSVFVKTQKATAGAEAEIVMFD 61
           ESCPSVKNILLLDSEGKRVAVKY++DDW + ++KLAFEKSVF KT K  A +EAEI +FD
Sbjct: 7   ESCPSVKNILLLDSEGKRVAVKYFSDDWSSNASKLAFEKSVFTKTLKTNARSEAEITLFD 66

Query: 62  GHIVVYKFIQDLHFFVTGGEEENELILASVLQGFTDAVDIILRNNVDKRTAXXXXXXXXX 121
           G+IVVYKF+QDLHFFVT G++ENELI+A+VLQGF+D+V ++LR +V+KRTA         
Sbjct: 67  GYIVVYKFVQDLHFFVTAGDDENELIIANVLQGFSDSVGLLLRGDVEKRTALENLDLILL 126

Query: 122 XXXXXVDGGIVLETEGSVIAEKVSAHGIEGATSLAEQTIVQALTTAREHLTKSLL 176
                VDGGI+LET+ + IA KV+ + ++G+   +EQTI QAL TAREHL +SLL
Sbjct: 127 CIDEIVDGGIILETDANTIAGKVATNAVDGSAPFSEQTISQALATAREHLARSLL 181



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.132    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,550,814
Number of extensions: 143517
Number of successful extensions: 271
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 271
Number of HSP's successfully gapped: 3
Length of query: 177
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 83
Effective length of database: 12,127,685
Effective search space: 1006597855
Effective search space used: 1006597855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 152 (63.2 bits)