BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0459300 Os05g0459300|AK121463
         (336 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0459300  Conserved hypothetical protein                      196   2e-50

>Os05g0459300 Conserved hypothetical protein
          Length = 336

 Score =  196 bits (499), Expect = 2e-50,   Method: Compositional matrix adjust.
 Identities = 102/145 (70%), Positives = 102/145 (70%)

Query: 192 PALAVHDPARRRQQEAHPGAHRQRDGPVQVQEGXXXXXXXXXXXXXXXXXXXXXXXQEDT 251
           PALAVHDPARRRQQEAHPGAHRQRDGPVQVQEG                       QEDT
Sbjct: 192 PALAVHDPARRRQQEAHPGAHRQRDGPVQVQEGLPHHLRLRRPPPRRRRLHVRRRPQEDT 251

Query: 252 RVRGGAQGFLGSHRHARHAQPEEHHLAVPGRSGXXXXXXXXXXXXXXXXXXXXACNLQRS 311
           RVRGGAQGFLGSHRHARHAQPEEHHLAVPGRSG                    ACNLQRS
Sbjct: 252 RVRGGAQGFLGSHRHARHAQPEEHHLAVPGRSGSLIQLSQVLLIVSSVQVLLLACNLQRS 311

Query: 312 LILFLRFNWFIPVSMFHFLCWKGVV 336
           LILFLRFNWFIPVSMFHFLCWKGVV
Sbjct: 312 LILFLRFNWFIPVSMFHFLCWKGVV 336




 Score =  101 bits (251), Expect = 9e-22,   Method: Compositional matrix adjust.
 Identities = 62/112 (55%), Positives = 62/112 (55%)

Query: 1   MVVPAATLKQQAQRGEEVRRXXXXXXGSSLQSADFSLGRRTAPLNSVASLXXXXXXXXXA 60
           MVVPAATLKQQAQRGEEVRR      GSSLQSADFSLGRRTAPLNSVASL         A
Sbjct: 1   MVVPAATLKQQAQRGEEVRRWWWWWWGSSLQSADFSLGRRTAPLNSVASLQQQRQQKQKA 60

Query: 61  GSHXXXXXXXXXXXXXXXXXXXXXXXHPRWGGALQXXXXXXXXXXXXPVHRE 112
           GSH                       HPRWGGALQ            PVHRE
Sbjct: 61  GSHGRGEEEGEGEEEEAQGEGEGEGGHPRWGGALQAERGGEGGAVRRPVHRE 112



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.135    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,890,211
Number of extensions: 131053
Number of successful extensions: 327
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 324
Number of HSP's successfully gapped: 2
Length of query: 336
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 235
Effective length of database: 11,762,187
Effective search space: 2764113945
Effective search space used: 2764113945
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 156 (64.7 bits)