BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0420600 Os05g0420600|AK060267
(112 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0420600 Cytochrome c 234 1e-62
Os01g0885000 Similar to Cytochrome c 174 1e-44
AK121277 148 1e-36
AK062980 142 6e-35
>Os05g0420600 Cytochrome c
Length = 112
Score = 234 bits (597), Expect = 1e-62, Method: Compositional matrix adjust.
Identities = 112/112 (100%), Positives = 112/112 (100%)
Query: 1 MASFSEAPPGNPKAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSTA 60
MASFSEAPPGNPKAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSTA
Sbjct: 1 MASFSEAPPGNPKAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSTA 60
Query: 61 NKNMAVIWEENTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLISYLKEATS 112
NKNMAVIWEENTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLISYLKEATS
Sbjct: 61 NKNMAVIWEENTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLISYLKEATS 112
>Os01g0885000 Similar to Cytochrome c
Length = 111
Score = 174 bits (441), Expect = 1e-44, Method: Compositional matrix adjust.
Identities = 82/112 (73%), Positives = 94/112 (83%), Gaps = 1/112 (0%)
Query: 1 MASFSEAPPGNPKAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSTA 60
MA+FS+APPG+ AGEKIF+TKCA CH VDK AGHK GPNLNGLFGRQSGT PG+SY +
Sbjct: 1 MATFSDAPPGDAAAGEKIFRTKCAYCHAVDKAAGHKHGPNLNGLFGRQSGTAPGFSYPSG 60
Query: 61 NKNMAVIWEENTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLISYLKEATS 112
+K + VIWEENTLYDYLL PKKY P KM F GLK+PQ+RADLI+YLK AT+
Sbjct: 61 DKIVPVIWEENTLYDYLLTPKKYTP-AKMGFNGLKQPQDRADLIAYLKNATA 111
>AK121277
Length = 95
Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust.
Identities = 66/82 (80%), Positives = 74/82 (90%)
Query: 1 MASFSEAPPGNPKAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSTA 60
MASF AP GN +G+KIF+TKCAQCHTV++G HKQGPNL+GLFGRQSGTTPGY+YSTA
Sbjct: 1 MASFGVAPVGNAASGKKIFRTKCAQCHTVERGGAHKQGPNLHGLFGRQSGTTPGYAYSTA 60
Query: 61 NKNMAVIWEENTLYDYLLNPKK 82
NKNMAV+WEE TLYDYLLNPKK
Sbjct: 61 NKNMAVVWEEGTLYDYLLNPKK 82
>AK062980
Length = 109
Score = 142 bits (358), Expect = 6e-35, Method: Compositional matrix adjust.
Identities = 62/102 (60%), Positives = 78/102 (76%)
Query: 10 GNPKAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSTANKNMAVIWE 69
GN K GEKIFK +C QCHT++ G HK GPNL+G++ RQSG GY YS AN V W
Sbjct: 6 GNAKNGEKIFKARCTQCHTINAGGSHKVGPNLHGMWNRQSGKAKGYDYSAANLERGVTWN 65
Query: 70 ENTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLISYLKEAT 111
+ TL++YL NPKKYIPGTKM+F GLKK +ER+DLI+Y+K+A+
Sbjct: 66 DETLFEYLENPKKYIPGTKMIFAGLKKDKERSDLIAYMKQAS 107
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.312 0.131 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,354,140
Number of extensions: 172031
Number of successful extensions: 256
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 255
Number of HSP's successfully gapped: 4
Length of query: 112
Length of database: 17,035,801
Length adjustment: 79
Effective length of query: 33
Effective length of database: 12,910,895
Effective search space: 426059535
Effective search space used: 426059535
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 149 (62.0 bits)