BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0386900 Os05g0386900|AK073634
         (352 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0386900  Reticulon family protein                            576   e-165
Os05g0513700  Reticulon family protein                             71   1e-12

>Os05g0386900 Reticulon family protein
          Length = 352

 Score =  576 bits (1485), Expect = e-165,   Method: Compositional matrix adjust.
 Identities = 288/352 (81%), Positives = 288/352 (81%)

Query: 1   MSLHQRPHQKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWGXXX 60
           MSLHQRPHQK                                             WG   
Sbjct: 1   MSLHQRPHQKPPATDSLPASAAAAAAAPSRPLPLLTLPYLFSLLALLLLLALLFPWGPPS 60

Query: 61  XXXXXXXXWRGYTLQEXXXXXXXXGNGTIVLAAVSGPYLPFLSNWLITVRRAGRADQVLV 120
                   WRGYTLQE        GNGTIVLAAVSGPYLPFLSNWLITVRRAGRADQVLV
Sbjct: 61  HSSAPASPWRGYTLQEAAAFAARAGNGTIVLAAVSGPYLPFLSNWLITVRRAGRADQVLV 120

Query: 121 VAEDYDTLERINAAWPGHAVLVPPAPDAQVAHKFGSQGFFNFTSRRPRHLLQILELGYSV 180
           VAEDYDTLERINAAWPGHAVLVPPAPDAQVAHKFGSQGFFNFTSRRPRHLLQILELGYSV
Sbjct: 121 VAEDYDTLERINAAWPGHAVLVPPAPDAQVAHKFGSQGFFNFTSRRPRHLLQILELGYSV 180

Query: 181 MYNDVDMVWLADPFPYIVGDHDVYFMDDMTPVKPLDHSHELPPPGKKGRTYICSCMIFLR 240
           MYNDVDMVWLADPFPYIVGDHDVYFMDDMTPVKPLDHSHELPPPGKKGRTYICSCMIFLR
Sbjct: 181 MYNDVDMVWLADPFPYIVGDHDVYFMDDMTPVKPLDHSHELPPPGKKGRTYICSCMIFLR 240

Query: 241 PTEGAKLLLRKWIEELKEQPWSKKQKANDQPAFNWALNKTAGQVDVYLLPQSAFPTGGLY 300
           PTEGAKLLLRKWIEELKEQPWSKKQKANDQPAFNWALNKTAGQVDVYLLPQSAFPTGGLY
Sbjct: 241 PTEGAKLLLRKWIEELKEQPWSKKQKANDQPAFNWALNKTAGQVDVYLLPQSAFPTGGLY 300

Query: 301 FKNKTWVKETKGKHVIIHNNYITGFEKKIKRFRDHGLWLVDEHSDESPLGRI 352
           FKNKTWVKETKGKHVIIHNNYITGFEKKIKRFRDHGLWLVDEHSDESPLGRI
Sbjct: 301 FKNKTWVKETKGKHVIIHNNYITGFEKKIKRFRDHGLWLVDEHSDESPLGRI 352



>Os05g0513700 Reticulon family protein
          Length = 689

 Score = 70.9 bits (172), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 73/272 (26%), Positives = 110/272 (40%), Gaps = 28/272 (10%)

Query: 86  NGTIVLAAVSGPYLPFLSNWLITVRRAGRADQVLVVAEDYDTLE-RINAAWPGHAVLVPP 144
           N ++VLA     Y   L +W+  +RR  R    +V A D +T E  +    P     + P
Sbjct: 402 NRSVVLAVAGASYRDMLMSWVCRLRRL-RVTNFVVCALDQETYEFSVLQGMPVSRDTLSP 460

Query: 145 APDAQVAHKFGSQGFFNFTSRRPRHLLQILELGYSVMYNDVDMVWLADPFPYIVGDHDVY 204
              +     FG+Q F   T  + R +L+IL LGY+V+ +DVD+ W  +P         V 
Sbjct: 461 NNVSFDDCHFGTQCFQQVTKVKSRIVLKILRLGYNVLLSDVDVYWFHNP---------VS 511

Query: 205 FMDDMTPVKPLDHSHELPPPGKKGRTYICSCMIFLRPTEGAKLLLRKWIEE------LKE 258
           F+  + P      S E    G        +   +   ++ A +   + I +      L E
Sbjct: 512 FLHSLGPGTFAAQSDEFNQTGPINMPRRLNSGFYYARSDDATITAMEMIVKHATNSGLSE 571

Query: 259 QP-----WSKKQKAN---DQPAFNWALNKTAGQVDVYLLPQSAFPTGGLYFKNKTWVKET 310
           QP        K  AN   D      + N T   +   + P  A+  GGL+ K        
Sbjct: 572 QPSFYDILCGKDGANRIGDDRCLEPSTNLTIVFLSRDMFPNGAY--GGLWEKKHGVSSAC 629

Query: 311 KG-KHVIIHNNYITGFEKKIKRFRDHGLWLVD 341
           +    VIIHNN++ G  KK+ R    GLW  D
Sbjct: 630 RELGCVIIHNNWVNGRRKKLHRQMASGLWDYD 661



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.139    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 11,712,155
Number of extensions: 509298
Number of successful extensions: 870
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 872
Number of HSP's successfully gapped: 2
Length of query: 352
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 250
Effective length of database: 11,709,973
Effective search space: 2927493250
Effective search space used: 2927493250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 156 (64.7 bits)