BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0202800 Os05g0202800|J065159M07
(99 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0202800 Conserved hypothetical protein 141 9e-35
AF009959 71 1e-13
Os01g0200700 Similar to Metallothionein-like protein type 3... 65 1e-11
>Os05g0202800 Conserved hypothetical protein
Length = 99
Score = 141 bits (356), Expect = 9e-35, Method: Compositional matrix adjust.
Identities = 72/99 (72%), Positives = 72/99 (72%)
Query: 1 MSDKCGNCDCADKSQCVKKGTSYGVVIVDAEKRATSRWLXXXXXXXXXXXXXXXXXXXXX 60
MSDKCGNCDCADKSQCVKKGTSYGVVIVDAEKRATSRWL
Sbjct: 1 MSDKCGNCDCADKSQCVKKGTSYGVVIVDAEKRATSRWLRRSATRRTTASASAPPAAPAP 60
Query: 61 XXXXXXDEQSYPLQPGPTIQCVIRYAPMRAYICICMHAC 99
DEQSYPLQPGPTIQCVIRYAPMRAYICICMHAC
Sbjct: 61 AATAASDEQSYPLQPGPTIQCVIRYAPMRAYICICMHAC 99
>AF009959
Length = 65
Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust.
Identities = 35/39 (89%), Positives = 37/39 (94%), Gaps = 1/39 (2%)
Query: 1 MSDKCGNCDCADKSQCVKKGTSYGVVIVDAEKRATSRWL 39
MSDKCGNCDCADKSQCVKKGTSYGVV+VDAEK TS+WL
Sbjct: 1 MSDKCGNCDCADKSQCVKKGTSYGVVLVDAEK-TTSKWL 38
>Os01g0200700 Similar to Metallothionein-like protein type 3 (MT-3) (MWMT3)
Length = 62
Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 31/32 (96%), Positives = 32/32 (100%)
Query: 1 MSDKCGNCDCADKSQCVKKGTSYGVVIVDAEK 32
MSDKCGNCDCADKSQCVKKGTSYGVVIV+AEK
Sbjct: 1 MSDKCGNCDCADKSQCVKKGTSYGVVIVEAEK 32
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.325 0.136 0.464
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,569,118
Number of extensions: 53853
Number of successful extensions: 132
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 131
Number of HSP's successfully gapped: 4
Length of query: 99
Length of database: 17,035,801
Length adjustment: 68
Effective length of query: 31
Effective length of database: 13,485,249
Effective search space: 418042719
Effective search space used: 418042719
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)