BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0181700 Os05g0181700|J065137K08
         (177 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0181700  Conserved hypothetical protein                      250   3e-67
Os05g0181600                                                      132   2e-31

>Os05g0181700 Conserved hypothetical protein
          Length = 177

 Score =  250 bits (639), Expect = 3e-67,   Method: Compositional matrix adjust.
 Identities = 128/177 (72%), Positives = 128/177 (72%)

Query: 1   MDNAKEEMRRAAATADSPDAGEPHAIAVHLQGWCRXXXXXXXXXXXXXXXVQTAGRARHD 60
           MDNAKEEMRRAAATADSPDAGEPHAIAVHLQGWCR               VQTAGRARHD
Sbjct: 1   MDNAKEEMRRAAATADSPDAGEPHAIAVHLQGWCRACLHLAALAFLACAFVQTAGRARHD 60

Query: 61  PWDXXXXXXXXXXXXXXXXXXRRAERLTPESPADDRRCLQRAAWALSTVLSCLFAYRVAR 120
           PWD                  RRAERLTPESPADDRRCLQRAAWALSTVLSCLFAYRVAR
Sbjct: 61  PWDLAFVVAAYASLAALFVVLRRAERLTPESPADDRRCLQRAAWALSTVLSCLFAYRVAR 120

Query: 121 IMPXXXXXXXXXXXXXXXXGGLYFLVLNDGDRGSEEDCHVADDGKLVFQKIPREEMV 177
           IMP                GGLYFLVLNDGDRGSEEDCHVADDGKLVFQKIPREEMV
Sbjct: 121 IMPAAMAVAVWAMTATVVAGGLYFLVLNDGDRGSEEDCHVADDGKLVFQKIPREEMV 177



>Os05g0181600 
          Length = 182

 Score =  132 bits (331), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 78/159 (49%), Positives = 85/159 (53%), Gaps = 3/159 (1%)

Query: 1   MDNAKEEMRR-AAATADSPDAGEPHAIAVHLQGWCRXXXXXXXXXXXXXXXVQTAGRARH 59
           MD+AKEEMRR AA TA SPDAGE  +IAVHLQGWCR               VQT   A  
Sbjct: 1   MDSAKEEMRRRAATTAGSPDAGEADSIAVHLQGWCRACLHLAALTFLACAFVQTTRPALS 60

Query: 60  DPWDXXXXXXXXXXXXXXXXXXRRAERLTPESPADDRRCLQRAAWALSTVLSCLFAYRVA 119
           DPWD                  RR ++LTP SPA +RR L R AW L T LSC FAYRV 
Sbjct: 61  DPWDLAFVVAAYANLAALFVVLRRVKQLTPMSPAKERRRLLRQAWELLTALSCAFAYRVE 120

Query: 120 RIMPXXXXXXXXXXXXXXXXGGLYFLVLNDGDRGSEEDC 158
           + MP                GG+YFLVLNDG RGS  DC
Sbjct: 121 QTMPAAMAVFVWAMTASVVVGGVYFLVLNDG-RGS-GDC 157



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.135    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,798,231
Number of extensions: 132556
Number of successful extensions: 410
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 406
Number of HSP's successfully gapped: 2
Length of query: 177
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 83
Effective length of database: 12,127,685
Effective search space: 1006597855
Effective search space used: 1006597855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 152 (63.2 bits)