BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0104800 Os05g0104800|AK070215
         (284 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0104800  Similar to Eukaryotic translation initiation f...   544   e-155
AK110114                                                          151   4e-37

>Os05g0104800 Similar to Eukaryotic translation initiation factor 3 subunit 5
           (eIF-3 epsilon) (eIF3 p32 subunit) (eIF3f)
          Length = 284

 Score =  544 bits (1401), Expect = e-155,   Method: Compositional matrix adjust.
 Identities = 269/284 (94%), Positives = 269/284 (94%)

Query: 1   MAAAEGPVLLFPSTSSTSARVEAVVVFNICDSYVRRPDQAERVIGTLLGSVLPDGTVHVR 60
           MAAAEGPVLLFPSTSSTSARVEAVVVFNICDSYVRRPDQAERVIGTLLGSVLPDGTVHVR
Sbjct: 1   MAAAEGPVLLFPSTSSTSARVEAVVVFNICDSYVRRPDQAERVIGTLLGSVLPDGTVHVR 60

Query: 61  NSYVVPHNESPDQVALDIEYHHNMYASHHKVNPKEVIXXXXXXXXXXXXXXXLIHDFYSR 120
           NSYVVPHNESPDQVALDIEYHHNMYASHHKVNPKEVI               LIHDFYSR
Sbjct: 61  NSYVVPHNESPDQVALDIEYHHNMYASHHKVNPKEVIVGWFSTGFGVSGGSTLIHDFYSR 120

Query: 121 EVQSPIHLTVDTGFTRGDASIKAYISSNLSLGDRHLAAQFQEIPLDLRMLEAGKVGFDIL 180
           EVQSPIHLTVDTGFTRGDASIKAYISSNLSLGDRHLAAQFQEIPLDLRMLEAGKVGFDIL
Sbjct: 121 EVQSPIHLTVDTGFTRGDASIKAYISSNLSLGDRHLAAQFQEIPLDLRMLEAGKVGFDIL 180

Query: 181 KSTIVEKLPNDLEGMESSMEKLYVLIDEIYKYVDDVVEGRVAPDNKIGRFISDAVASMPK 240
           KSTIVEKLPNDLEGMESSMEKLYVLIDEIYKYVDDVVEGRVAPDNKIGRFISDAVASMPK
Sbjct: 181 KSTIVEKLPNDLEGMESSMEKLYVLIDEIYKYVDDVVEGRVAPDNKIGRFISDAVASMPK 240

Query: 241 LSPAAFDKLFNDRIQDNLALVYLSSITRTQISIAEKLNTAAQIL 284
           LSPAAFDKLFNDRIQDNLALVYLSSITRTQISIAEKLNTAAQIL
Sbjct: 241 LSPAAFDKLFNDRIQDNLALVYLSSITRTQISIAEKLNTAAQIL 284



>AK110114 
          Length = 348

 Score =  151 bits (382), Expect = 4e-37,   Method: Compositional matrix adjust.
 Identities = 93/291 (31%), Positives = 156/291 (53%), Gaps = 17/291 (5%)

Query: 7   PVLLFPSTSST---SARVEAVVVFNICDSYVRRPDQAERVIGTLLGSVLPDGT-VHVRNS 62
           P  +  STS+T   S  ++   +F+I D   RRP   ERVIGTLLG+   DGT V +RN 
Sbjct: 15  PAQISQSTSTTAPCSVTIQPQALFSILDHASRRPADQERVIGTLLGTRSEDGTEVEIRNC 74

Query: 63  YVVPHNESPDQVALDIEYHHNMYASHHKVNPKEVIXXXXXXXXXXXXXXXLIHDFYSREV 122
           Y VPH E+ +QV +D++Y  +M   H +  PKEV+               LI +FYS++ 
Sbjct: 75  YAVPHIETAEQVEVDMDYQKSMLQLHQRAAPKEVLVGWYATSDELNTFTALIQNFYSQQG 134

Query: 123 QS-----PIHLTVDTGFTRGDASIKAYISSNLSLGDRHLA--AQFQEIPLDLRMLEAGKV 175
           +       +HLTV T     D +   YIS+ + +     A    F  +P +++  +A K 
Sbjct: 135 EGTWPHPAVHLTVST-VAGKDIAANTYISAPIGVTPERAADSCLFIPVPNEIKYGDAEKS 193

Query: 176 GFDIL-----KSTIVEKLPNDLEGMESSMEKLYVLIDEIYKYVDDVVEGRVAPDNKIGRF 230
           G +++     +    + L  D+E +E ++E++  +++ +  YV +V++    P + +G+F
Sbjct: 194 GLELIAGAREREDRTQLLQTDIETLERAIEQVLEMLERVSNYVSNVLDEEAEPSSALGQF 253

Query: 231 ISDAVASMPKLSPAAFDKLFNDRIQDNLALVYLSSITRTQISIAEKLNTAA 281
           + + +A  PK+ P   ++ FN+ IQD L + YLS+  RTQI ++ +L  AA
Sbjct: 254 LMNTLALAPKVEPEQVERDFNNHIQDVLLVSYLSNTIRTQIDLSNRLAIAA 304



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.134    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,290,550
Number of extensions: 318010
Number of successful extensions: 791
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 789
Number of HSP's successfully gapped: 2
Length of query: 284
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 184
Effective length of database: 11,814,401
Effective search space: 2173849784
Effective search space used: 2173849784
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 155 (64.3 bits)