BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0458400 Os04g0458400|AK070570
(93 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0458400 Conserved hypothetical protein 149 5e-37
Os12g0537700 87 2e-18
Os12g0538033 87 2e-18
Os12g0537900 87 2e-18
Os03g0672000 83 3e-17
Os09g0280400 64 3e-11
>Os04g0458400 Conserved hypothetical protein
Length = 93
Score = 149 bits (376), Expect = 5e-37, Method: Compositional matrix adjust.
Identities = 74/93 (79%), Positives = 74/93 (79%)
Query: 1 MASALWXXXXXXXXXXXXXXXXXXXWHSRSGARSKDIPGCTKFIKTGLQNEELLEKMFED 60
MASALW WHSRSGARSKDIPGCTKFIKTGLQNEELLEKMFED
Sbjct: 1 MASALWRRERLPTARRRTAGAGRRRWHSRSGARSKDIPGCTKFIKTGLQNEELLEKMFED 60
Query: 61 IRNTGANHWSLGQGTIPTAMDAFRDYLAGRIAG 93
IRNTGANHWSLGQGTIPTAMDAFRDYLAGRIAG
Sbjct: 61 IRNTGANHWSLGQGTIPTAMDAFRDYLAGRIAG 93
>Os12g0537700
Length = 297
Score = 87.4 bits (215), Expect = 2e-18, Method: Composition-based stats.
Identities = 38/43 (88%), Positives = 41/43 (95%)
Query: 36 DIPGCTKFIKTGLQNEELLEKMFEDIRNTGANHWSLGQGTIPT 78
DIPGCTKF+K GLQNEELL+KMFEDIRNTGA+HWSLGQ TIPT
Sbjct: 115 DIPGCTKFMKAGLQNEELLQKMFEDIRNTGADHWSLGQSTIPT 157
>Os12g0538033
Length = 546
Score = 87.4 bits (215), Expect = 2e-18, Method: Composition-based stats.
Identities = 38/43 (88%), Positives = 41/43 (95%)
Query: 36 DIPGCTKFIKTGLQNEELLEKMFEDIRNTGANHWSLGQGTIPT 78
DIPGCTKF+K GLQNEELL+KMFEDIRNTGA+HWSLGQ TIPT
Sbjct: 115 DIPGCTKFMKAGLQNEELLQKMFEDIRNTGADHWSLGQSTIPT 157
>Os12g0537900
Length = 546
Score = 87.4 bits (215), Expect = 2e-18, Method: Composition-based stats.
Identities = 38/43 (88%), Positives = 41/43 (95%)
Query: 36 DIPGCTKFIKTGLQNEELLEKMFEDIRNTGANHWSLGQGTIPT 78
DIPGCTKF+K GLQNEELL+KMFEDIRNTGA+HWSLGQ TIPT
Sbjct: 115 DIPGCTKFMKAGLQNEELLQKMFEDIRNTGADHWSLGQSTIPT 157
>Os03g0672000
Length = 298
Score = 83.2 bits (204), Expect = 3e-17, Method: Composition-based stats.
Identities = 38/45 (84%), Positives = 41/45 (91%)
Query: 35 KDIPGCTKFIKTGLQNEELLEKMFEDIRNTGANHWSLGQGTIPTA 79
+DI GC KF K GLQNEELLEKMFEDIRNTGA+HWSLGQGTIP+A
Sbjct: 114 EDIAGCLKFRKAGLQNEELLEKMFEDIRNTGADHWSLGQGTIPSA 158
>Os09g0280400
Length = 93
Score = 63.5 bits (153), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 28/33 (84%), Positives = 29/33 (87%)
Query: 36 DIPGCTKFIKTGLQNEELLEKMFEDIRNTGANH 68
DI GCTKF+K GLQNEELLEKMFEDI NT ANH
Sbjct: 61 DIAGCTKFMKVGLQNEELLEKMFEDIHNTDANH 93
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.133 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,411,743
Number of extensions: 79709
Number of successful extensions: 206
Number of sequences better than 1.0e-10: 6
Number of HSP's gapped: 206
Number of HSP's successfully gapped: 6
Length of query: 93
Length of database: 17,035,801
Length adjustment: 62
Effective length of query: 31
Effective length of database: 13,798,533
Effective search space: 427754523
Effective search space used: 427754523
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)