BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0269700 Os04g0269700|AK069600
         (209 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0269700  Lipolytic enzyme, G-D-S-L family protein            295   1e-80
Os01g0214200  Lipolytic enzyme, G-D-S-L family protein            126   1e-29
Os09g0121900                                                      118   4e-27
Os06g0156400  Lipolytic enzyme, G-D-S-L family protein             69   2e-12
Os10g0476700  Lipolytic enzyme, G-D-S-L family protein             66   2e-11

>Os04g0269700 Lipolytic enzyme, G-D-S-L family protein
          Length = 209

 Score =  295 bits (756), Expect = 1e-80,   Method: Compositional matrix adjust.
 Identities = 157/209 (75%), Positives = 157/209 (75%)

Query: 1   SALTLLSRSPSKSVLPPSRVRSFCFLPIDSPRSASDLRGSDTLPPYPLLSGRRPIPTCVR 60
           SALTLLSRSPSKSVLPPSRVRSFCFLPIDSPRSASDLRGSDTLPPYPLLSGRRPIPTCVR
Sbjct: 1   SALTLLSRSPSKSVLPPSRVRSFCFLPIDSPRSASDLRGSDTLPPYPLLSGRRPIPTCVR 60

Query: 61  HRFLERPTWCCAGSAATTXXXXXXXXXXXXXXXXXXXXQTRWLSRCSWAPXXXXXXXXXX 120
           HRFLERPTWCCAGSAATT                    QTRWLSRCSWAP          
Sbjct: 61  HRFLERPTWCCAGSAATTRGGRCGCWRGRWRVPPPWGRQTRWLSRCSWAPTTRRCRIGSR 120

Query: 121 XXXXXXXXXXXXXXXXXXXXXXDMYGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVI 180
                                 DMYGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVI
Sbjct: 121 CTSTCRSTSTRATSAPSAPTSRDMYGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVI 180

Query: 181 DIWTKMQQFPDWQTSALCRGLEAKPTVWR 209
           DIWTKMQQFPDWQTSALCRGLEAKPTVWR
Sbjct: 181 DIWTKMQQFPDWQTSALCRGLEAKPTVWR 209



>Os01g0214200 Lipolytic enzyme, G-D-S-L family protein
          Length = 238

 Score =  126 bits (317), Expect = 1e-29,   Method: Compositional matrix adjust.
 Identities = 58/63 (92%), Positives = 58/63 (92%)

Query: 143 DMYGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALCRGLE 202
           DMYGEDDPSKLPERTNEA GTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALC GL 
Sbjct: 131 DMYGEDDPSKLPERTNEAAGTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALCDGLH 190

Query: 203 AKP 205
             P
Sbjct: 191 FTP 193



>Os09g0121900 
          Length = 132

 Score =  118 bits (295), Expect = 4e-27,   Method: Compositional matrix adjust.
 Identities = 55/58 (94%), Positives = 55/58 (94%)

Query: 143 DMYGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALCRG 200
           DMYGEDDPSKLPERTNEA GTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSAL  G
Sbjct: 48  DMYGEDDPSKLPERTNEAAGTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALWYG 105



>Os06g0156400 Lipolytic enzyme, G-D-S-L family protein
          Length = 189

 Score = 68.9 bits (167), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 34/61 (55%), Positives = 42/61 (68%), Gaps = 1/61 (1%)

Query: 145 YGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALCRGLEAK 204
           YG  D + LPERTNE+ G YA+AC+ VA E    VIDIW+KMQ+FP W++S L  GL   
Sbjct: 85  YG-GDCAGLPERTNESAGAYARACVEVAAECGLRVIDIWSKMQRFPGWESSFLRDGLHLT 143

Query: 205 P 205
           P
Sbjct: 144 P 144



>Os10g0476700 Lipolytic enzyme, G-D-S-L family protein
          Length = 270

 Score = 66.2 bits (160), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 31/62 (50%), Positives = 39/62 (62%), Gaps = 1/62 (1%)

Query: 144 MYGEDDPSKLPERTNEATGTYAQACLTVAKELNHPVIDIWTKMQQFPDWQTSALCRGLEA 203
           +YGE+   KLPERTNE  G YA  C+ +A+E+N   IDIW+KMQ+   WQ   L  GL  
Sbjct: 166 LYGEE-ARKLPERTNEMAGVYASQCIELAREMNIHCIDIWSKMQETAGWQKLYLSDGLHL 224

Query: 204 KP 205
            P
Sbjct: 225 TP 226



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.132    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,311,535
Number of extensions: 222366
Number of successful extensions: 535
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 533
Number of HSP's successfully gapped: 5
Length of query: 209
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 113
Effective length of database: 12,023,257
Effective search space: 1358628041
Effective search space used: 1358628041
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)