BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0227100 Os04g0227100|AK120507
         (124 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0227100  Conserved hypothetical protein                      241   1e-64
Os04g0226700                                                       74   2e-14
>Os04g0227100 Conserved hypothetical protein
          Length = 124

 Score =  241 bits (614), Expect = 1e-64,   Method: Compositional matrix adjust.
 Identities = 124/124 (100%), Positives = 124/124 (100%)

Query: 1   MELRDEIFARDGGILPESKFPVRSRNRRVPMLRGSGILPTKEFVCRSTVTSLDEPLKKLG 60
           MELRDEIFARDGGILPESKFPVRSRNRRVPMLRGSGILPTKEFVCRSTVTSLDEPLKKLG
Sbjct: 1   MELRDEIFARDGGILPESKFPVRSRNRRVPMLRGSGILPTKEFVCRSTVTSLDEPLKKLG 60

Query: 61  GISPDRLLRERFSSMSDELLAKDSGMVPSRLFLASSRLCREVRLPMQSGRTPVSSMELRF 120
           GISPDRLLRERFSSMSDELLAKDSGMVPSRLFLASSRLCREVRLPMQSGRTPVSSMELRF
Sbjct: 61  GISPDRLLRERFSSMSDELLAKDSGMVPSRLFLASSRLCREVRLPMQSGRTPVSSMELRF 120

Query: 121 METT 124
           METT
Sbjct: 121 METT 124
>Os04g0226700 
          Length = 308

 Score = 74.3 bits (181), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 35/44 (79%), Positives = 41/44 (93%)

Query: 75  MSDELLAKDSGMVPSRLFLASSRLCREVRLPMQSGRTPVSSMEL 118
           MSD+LLAKDSGMVPSRLFL+ +R+C EVRLPMQ+GRTP+S MEL
Sbjct: 1   MSDKLLAKDSGMVPSRLFLSRTRVCNEVRLPMQAGRTPLSFMEL 44
  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.321    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,201,755
Number of extensions: 168092
Number of successful extensions: 422
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 419
Number of HSP's successfully gapped: 3
Length of query: 124
Length of database: 17,035,801
Length adjustment: 88
Effective length of query: 36
Effective length of database: 12,440,969
Effective search space: 447874884
Effective search space used: 447874884
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)