BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0183300 Os04g0183300|AK100045
         (210 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0183300  Amidase family protein                              172   1e-43
Os04g0182900  Amidase family protein                               97   8e-21
Os04g0183500  Amidase family protein                               89   2e-18
Os04g0184100  Amidase family protein                               83   1e-16
Os04g0184500  Amidase family protein                               80   1e-15

>Os04g0183300 Amidase family protein
          Length = 210

 Score =  172 bits (437), Expect = 1e-43,   Method: Compositional matrix adjust.
 Identities = 89/110 (80%), Positives = 89/110 (80%)

Query: 101 CCGGLHGVPVLLKDSIATRDRLNTTAGSLALLGSXXXXXXXXXXXXXXXXXXXXXKANLD 160
           CCGGLHGVPVLLKDSIATRDRLNTTAGSLALLGS                     KANLD
Sbjct: 101 CCGGLHGVPVLLKDSIATRDRLNTTAGSLALLGSVVRRDAGVVRRLRRAGAVVLGKANLD 160

Query: 161 EWANFRTIQGTGGWSARGGQSRVRLSYLLSLANFAIGFSITKVNAEYRSL 210
           EWANFRTIQGTGGWSARGGQSRVRLSYLLSLANFAIGFSITKVNAEYRSL
Sbjct: 161 EWANFRTIQGTGGWSARGGQSRVRLSYLLSLANFAIGFSITKVNAEYRSL 210



>Os04g0182900 Amidase family protein
          Length = 507

 Score = 97.1 bits (240), Expect = 8e-21,   Method: Compositional matrix adjust.
 Identities = 53/89 (59%), Positives = 57/89 (64%), Gaps = 2/89 (2%)

Query: 102 CGGLHGVPVLLKDSIATRDRLNTTAGSLALLGSXXXXXXXXXXXXXXXXXXXXXKANLDE 161
           CG LHGVPVLLKD IATRDRLNTTAGSL+LLG+                     KANL E
Sbjct: 91  CGPLHGVPVLLKDIIATRDRLNTTAGSLSLLGAVARRDAGVVARLRRAGAVVLGKANLPE 150

Query: 162 WANFRTIQGTGGWSARGGQSRVRLSYLLS 190
           WANFR+  G  GWSARGGQSR    Y+LS
Sbjct: 151 WANFRSSPGLRGWSARGGQSRN--PYVLS 177



>Os04g0183500 Amidase family protein
          Length = 511

 Score = 89.0 bits (219), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 46/82 (56%), Positives = 54/82 (65%), Gaps = 2/82 (2%)

Query: 102 CGGLHGVPVLLKDSIATRDRLNTTAGSLALLGSXXXXXXXXXXXXXXXXXXXXXKANLDE 161
           CG +HGVPVLLKD++ATRDRLNTTAGSLALLGS                     KA+L E
Sbjct: 87  CGPMHGVPVLLKDNMATRDRLNTTAGSLALLGSIVRRDAGVAARLRAAGAVILGKASLSE 146

Query: 162 WANFRTIQGTGGWSARGGQSRV 183
           W+NFR ++   GWSARGGQ+ V
Sbjct: 147 WSNFRPVK--SGWSARGGQTVV 166



>Os04g0184100 Amidase family protein
          Length = 524

 Score = 82.8 bits (203), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 44/80 (55%), Positives = 49/80 (61%), Gaps = 2/80 (2%)

Query: 102 CGGLHGVPVLLKDSIATRDRLNTTAGSLALLGSXXXXXXXXXXXXXXXXXXXXXKANLDE 161
           CG LHGVPVLLKD+IATRDRLNTTAGS ALLGS                     KA+L E
Sbjct: 108 CGPLHGVPVLLKDNIATRDRLNTTAGSFALLGSVVPRDAGVAARLRAAGAVILGKASLTE 167

Query: 162 WANFRTIQGTGGWSARGGQS 181
           W+ +R      GWSARGGQ+
Sbjct: 168 WSAYRP--APNGWSARGGQT 185



>Os04g0184500 Amidase family protein
          Length = 182

 Score = 79.7 bits (195), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 44/79 (55%), Positives = 51/79 (64%), Gaps = 2/79 (2%)

Query: 103 GGLHGVPVLLKDSIATRDRLNTTAGSLALLGSXXXXXXXXXXXXXXXXXXXXXKANLDEW 162
           G LHGVPVLLKD+IATRDRLNTTAGSLALLGS                     K+N  EW
Sbjct: 81  GPLHGVPVLLKDNIATRDRLNTTAGSLALLGSVVRCDAGVAARLRAAGAVILGKSNPAEW 140

Query: 163 ANFRTIQGTGGWSARGGQS 181
           ++FR +  T GWSARGG++
Sbjct: 141 SSFRPL--TNGWSARGGET 157



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.134    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,206,955
Number of extensions: 73649
Number of successful extensions: 168
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 165
Number of HSP's successfully gapped: 5
Length of query: 210
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 114
Effective length of database: 12,023,257
Effective search space: 1370651298
Effective search space used: 1370651298
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)