BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0105300 Os04g0105300|AK060144
(287 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0105300 Protein of unknown function DUF1295 family pro... 484 e-137
Os03g0708400 Protein of unknown function DUF1295 family pro... 69 4e-12
>Os04g0105300 Protein of unknown function DUF1295 family protein
Length = 287
Score = 484 bits (1247), Expect = e-137, Method: Compositional matrix adjust.
Identities = 241/287 (83%), Positives = 241/287 (83%)
Query: 1 TPLNSQQEEAEASIQSNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 60
TPLNSQQEEAEASIQSN
Sbjct: 1 TPLNSQQEEAEASIQSNRGHGRRREEHGHRHPRPSPFPPLLLLFRPPHPAAVADTARSAV 60
Query: 61 XXXLTWVWSARLTHNYLRREGWQWGKREDWRFAEMRGQYGRAWWWMSFFAVYLSQQVFLI 120
LTWVWSARLTHNYLRREGWQWGKREDWRFAEMRGQYGRAWWWMSFFAVYLSQQVFLI
Sbjct: 61 AVALTWVWSARLTHNYLRREGWQWGKREDWRFAEMRGQYGRAWWWMSFFAVYLSQQVFLI 120
Query: 121 GICLPMYAIHSTTQPWGAWDVVATMACLAGIVIAHFADTQLHRFVTTNEKLKKVGEPTVL 180
GICLPMYAIHSTTQPWGAWDVVATMACLAGIVIAHFADTQLHRFVTTNEKLKKVGEPTVL
Sbjct: 121 GICLPMYAIHSTTQPWGAWDVVATMACLAGIVIAHFADTQLHRFVTTNEKLKKVGEPTVL 180
Query: 181 TMEAGLWRYSRHPNYFGEQLWWWGLYLFAWNIGQPWMVVGPLVNSLCLGYVTVLVERRMV 240
TMEAGLWRYSRHPNYFGEQLWWWGLYLFAWNIGQPWMVVGPLVNSLCLGYVTVLVERRMV
Sbjct: 181 TMEAGLWRYSRHPNYFGEQLWWWGLYLFAWNIGQPWMVVGPLVNSLCLGYVTVLVERRMV 240
Query: 241 KQEHRAEAYKLYQKRTSVWIPWFRKPVPQPYNHKDSSNQNSLKISHS 287
KQEHRAEAYKLYQKRTSVWIPWFRKPVPQPYNHKDSSNQNSLKISHS
Sbjct: 241 KQEHRAEAYKLYQKRTSVWIPWFRKPVPQPYNHKDSSNQNSLKISHS 287
>Os03g0708400 Protein of unknown function DUF1295 family protein
Length = 291
Score = 68.9 bits (167), Expect = 4e-12, Method: Compositional matrix adjust.
Identities = 66/206 (32%), Positives = 100/206 (48%), Gaps = 25/206 (12%)
Query: 64 LTWVWSARLTHNYLRREGWQWGKREDWRFAEMRGQYGRAWWWMSFFAVY-LSQQVFLIGI 122
L +W RL L R QWG ED RF EMR G+ AV+ + Q V++ +
Sbjct: 69 LVVIWGLRLGLFLLMRI-LQWG--EDKRFDEMRDNLGK-------LAVFWIFQAVWVWTV 118
Query: 123 CLPMYAIH-STTQP-WGAWDVVATMACLAGIVIAHFADTQLHRFVTTNEKLKKVGEPTVL 180
LP+ ++ S + P A D++ + L G + AD Q F + +
Sbjct: 119 SLPVTIVNASDSDPSIEARDIIGWIMWLIGAGMEAIADQQKLTFKNSPSNRGR------- 171
Query: 181 TMEAGLWRYSRHPNYFGEQLWWWGLYLFAWNI--GQPWMVV-GPLVNSLCLGYVT--VLV 235
GLW Y+RHPNYFGE WWG+++ + + G W+V+ GP+ +L L +V+ L+
Sbjct: 172 WCNVGLWSYTRHPNYFGEIFLWWGIFVASTPVLSGAEWLVILGPVFLTLLLLFVSGIPLL 231
Query: 236 ERRMVKQEHRAEAYKLYQKRTSVWIP 261
E K+ + E Y+ Y+ TS IP
Sbjct: 232 EASADKRFGQNEEYRTYKNTTSPLIP 257
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.323 0.134 0.472
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,686,080
Number of extensions: 345470
Number of successful extensions: 793
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 791
Number of HSP's successfully gapped: 2
Length of query: 287
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 187
Effective length of database: 11,814,401
Effective search space: 2209292987
Effective search space used: 2209292987
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 155 (64.3 bits)