BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0850300 Os03g0850300|Os03g0850300
         (256 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0850300  Sterile alpha motif SAM domain containing protein   306   9e-84
Os12g0478900  Sterile alpha motif homology domain containing...    83   2e-16

>Os03g0850300 Sterile alpha motif SAM domain containing protein
          Length = 256

 Score =  306 bits (784), Expect = 9e-84,   Method: Compositional matrix adjust.
 Identities = 171/256 (66%), Positives = 171/256 (66%)

Query: 1   MADLHPPEPDTNGGSXXXXXXXXXXXXPLXXXXXXXXXXXXXXXXXYSKRRRRPSVRLGD 60
           MADLHPPEPDTNGGS            PL                 YSKRRRRPSVRLGD
Sbjct: 1   MADLHPPEPDTNGGSAAAAAAAAAAAAPLGDPAAEASGAQAAAGPPYSKRRRRPSVRLGD 60

Query: 61  IGVHSAAAADVPLPQXXXXXXXXXXXXXXXXXXXXEDALDPLNHSSAAAHRRGQKPAQRR 120
           IGVHSAAAADVPLPQ                    EDALDPLNHSSAAAHRRGQKPAQRR
Sbjct: 61  IGVHSAAAADVPLPQRRHHKTSSSSHPRPPRRAHPEDALDPLNHSSAAAHRRGQKPAQRR 120

Query: 121 PRTAWIPAAPGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDESDGVADWGL 180
           PRTAWIPAAPGT                                    GDESDGVADWGL
Sbjct: 121 PRTAWIPAAPGTDGYEEEAEHYYDDADQSDSAAAAAARARVSGSRDASGDESDGVADWGL 180

Query: 181 PNGRLPGSMGYGGVKAWLDGLGLSRYAPVFEIHEVDDEVLPLLTLEDLKDMGIGAVGSRR 240
           PNGRLPGSMGYGGVKAWLDGLGLSRYAPVFEIHEVDDEVLPLLTLEDLKDMGIGAVGSRR
Sbjct: 181 PNGRLPGSMGYGGVKAWLDGLGLSRYAPVFEIHEVDDEVLPLLTLEDLKDMGIGAVGSRR 240

Query: 241 KLYAAIQKLQRSDSVS 256
           KLYAAIQKLQRSDSVS
Sbjct: 241 KLYAAIQKLQRSDSVS 256



>Os12g0478900 Sterile alpha motif homology domain containing protein
          Length = 218

 Score = 83.2 bits (204), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 38/55 (69%), Positives = 45/55 (81%)

Query: 194 VKAWLDGLGLSRYAPVFEIHEVDDEVLPLLTLEDLKDMGIGAVGSRRKLYAAIQK 248
           V  WL G+G+ RYA  FE HEVD EVLP LT++DL+DMGIGAVG+RRKLY AIQ+
Sbjct: 147 VVEWLWGIGMGRYAAAFEAHEVDGEVLPCLTMDDLRDMGIGAVGARRKLYCAIQR 201



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.136    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,517,100
Number of extensions: 239799
Number of successful extensions: 481
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 478
Number of HSP's successfully gapped: 2
Length of query: 256
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 157
Effective length of database: 11,866,615
Effective search space: 1863058555
Effective search space used: 1863058555
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 155 (64.3 bits)