BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0840900 Os03g0840900|J080063E22
         (126 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0840900  Protein of unknown function DUF543 family protein   139   5e-34

>Os03g0840900 Protein of unknown function DUF543 family protein
          Length = 126

 Score =  139 bits (350), Expect = 5e-34,   Method: Compositional matrix adjust.
 Identities = 74/126 (58%), Positives = 74/126 (58%)

Query: 1   MAESPENXXXXXXXXXXXXXXXXXXXXXXXXXXKSGIPPRYDLDAKWDACLDISIRRVAY 60
           MAESPEN                          KSGIPPRYDLDAKWDACLDISIRRVAY
Sbjct: 1   MAESPENAAPAAAPAPAPAPTPAPPPPPSSPPTKSGIPPRYDLDAKWDACLDISIRRVAY 60

Query: 61  SXXXXXXXXXXXXRSPTTRWXXXXXXXXXXXXXXYTECSYLFNGAPPKWSPKVSTVPSAH 120
           S            RSPTTRW              YTECSYLFNGAPPKWSPKVSTVPSAH
Sbjct: 61  STLGGTFAGLLLFRSPTTRWASVALGAGVGIGAAYTECSYLFNGAPPKWSPKVSTVPSAH 120

Query: 121 SEGEDK 126
           SEGEDK
Sbjct: 121 SEGEDK 126



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.129    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,202,962
Number of extensions: 73518
Number of successful extensions: 93
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 93
Number of HSP's successfully gapped: 1
Length of query: 126
Length of database: 17,035,801
Length adjustment: 89
Effective length of query: 37
Effective length of database: 12,388,755
Effective search space: 458383935
Effective search space used: 458383935
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)