BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0838500 Os03g0838500|AK121130
         (116 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0838500  Proteinase inhibitor I9, subtilisin propeptide...   177   2e-45
Os07g0438600  Proteinase inhibitor, propeptide domain contai...    82   1e-16
Os05g0432700  Proteinase inhibitor I9, subtilisin propeptide...    73   5e-14

>Os03g0838500 Proteinase inhibitor I9, subtilisin propeptide domain containing
           protein
          Length = 116

 Score =  177 bits (448), Expect = 2e-45,   Method: Compositional matrix adjust.
 Identities = 91/116 (78%), Positives = 91/116 (78%)

Query: 1   MKPWTXXXXXXXXXXXXXXXXXXXXXXXXXNYLVFVDPPPSGVVCTAYQLSILAAALGSE 60
           MKPWT                         NYLVFVDPPPSGVVCTAYQLSILAAALGSE
Sbjct: 1   MKPWTATVSLLLAAAVAMAAAVAAGEGGAANYLVFVDPPPSGVVCTAYQLSILAAALGSE 60

Query: 61  EKAKGAIIYNYKNVVSGFSARLTPSELEAVKKQPQVNRVLPSATLSLMSSNFDGIS 116
           EKAKGAIIYNYKNVVSGFSARLTPSELEAVKKQPQVNRVLPSATLSLMSSNFDGIS
Sbjct: 61  EKAKGAIIYNYKNVVSGFSARLTPSELEAVKKQPQVNRVLPSATLSLMSSNFDGIS 116



>Os07g0438600 Proteinase inhibitor, propeptide domain containing protein
          Length = 117

 Score = 81.6 bits (200), Expect = 1e-16,   Method: Compositional matrix adjust.
 Identities = 39/71 (54%), Positives = 50/71 (70%)

Query: 32  YLVFVDPPPSGVVCTAYQLSILAAALGSEEKAKGAIIYNYKNVVSGFSARLTPSELEAVK 91
           Y+V V PP  G    AY   ILAAALGSEE+AK A++Y+Y+ V SGF+A+LTP EL A++
Sbjct: 37  YIVIVQPPADGADTVAYHTCILAAALGSEERAKEALLYSYRAVASGFAAKLTPPELSALQ 96

Query: 92  KQPQVNRVLPS 102
           K P V +V P 
Sbjct: 97  KHPAVLQVRPD 107



>Os05g0432700 Proteinase inhibitor I9, subtilisin propeptide domain containing
           protein
          Length = 109

 Score = 72.8 bits (177), Expect = 5e-14,   Method: Compositional matrix adjust.
 Identities = 36/75 (48%), Positives = 52/75 (69%), Gaps = 1/75 (1%)

Query: 33  LVFVDPPPSGVVCTAYQLSILAAALGSEEKAKGAIIYNYKNVVSGFSARLTPSELEAVKK 92
           +V++D P +      + +  LA  LGSEEKAK A++Y+YK+  SGFSA+LTP ++E +KK
Sbjct: 20  IVYLDRP-ADADPEEFHIRTLAPVLGSEEKAKEAVLYHYKHAASGFSAKLTPQQVEELKK 78

Query: 93  QPQVNRVLPSATLSL 107
           QP V +V+PS T  L
Sbjct: 79  QPGVLQVVPSQTYQL 93



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.129    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,096,224
Number of extensions: 93008
Number of successful extensions: 240
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 241
Number of HSP's successfully gapped: 3
Length of query: 116
Length of database: 17,035,801
Length adjustment: 83
Effective length of query: 33
Effective length of database: 12,702,039
Effective search space: 419167287
Effective search space used: 419167287
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 149 (62.0 bits)