BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os03g0822400 Os03g0822400|AK070075
(89 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os03g0822400 Conserved hypothetical protein 97 2e-21
Os01g0843700 Conserved hypothetical protein 66 5e-12
>Os03g0822400 Conserved hypothetical protein
Length = 89
Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust.
Identities = 52/70 (74%), Positives = 52/70 (74%)
Query: 20 LQGWETPRREECXXXXXXXXXXXXXXXXXXALPELGKERREPPKGGYFQPPDLESLFVLA 79
LQGWETPRREEC ALPELGKERREPPKGGYFQPPDLESLFVLA
Sbjct: 20 LQGWETPRREECRIPVVPPQCPAPPRKRPVALPELGKERREPPKGGYFQPPDLESLFVLA 79
Query: 80 PPRRQASSCA 89
PPRRQASSCA
Sbjct: 80 PPRRQASSCA 89
>Os01g0843700 Conserved hypothetical protein
Length = 93
Score = 66.2 bits (160), Expect = 5e-12, Method: Compositional matrix adjust.
Identities = 40/68 (58%), Positives = 44/68 (64%), Gaps = 6/68 (8%)
Query: 22 GWETPRREECXXXXXXXXXXXXXXXXXXALPELGKERREPPKGGYFQPPDLESLFVLAPP 81
GWETP+REEC A+P+ GK RR PPK GYFQPPDLE+LF LA P
Sbjct: 32 GWETPKREECRIPATLPCPAAPRK----AVPDFGK-RRGPPKNGYFQPPDLEALFALA-P 85
Query: 82 RRQASSCA 89
RRQASSCA
Sbjct: 86 RRQASSCA 93
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.138 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,714,462
Number of extensions: 84663
Number of successful extensions: 140
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 136
Number of HSP's successfully gapped: 2
Length of query: 89
Length of database: 17,035,801
Length adjustment: 59
Effective length of query: 30
Effective length of database: 13,955,175
Effective search space: 418655250
Effective search space used: 418655250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 149 (62.0 bits)