BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0748700 Os03g0748700|AK067853
         (476 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0748700  Iron hydrogenase domain containing protein          941   0.0  

>Os03g0748700 Iron hydrogenase domain containing protein
          Length = 476

 Score =  941 bits (2432), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 456/466 (97%), Positives = 456/466 (97%)

Query: 11  RFSPALQASDLNDFIAPSQDCIISLNKGPSARRLPIKQKEIAVSTNPPEEAVKISLKDCL 70
           RFSPALQASDLNDFIAPSQDCIISLNKGPSARRLPIKQKEIAVSTNPPEEAVKISLKDCL
Sbjct: 11  RFSPALQASDLNDFIAPSQDCIISLNKGPSARRLPIKQKEIAVSTNPPEEAVKISLKDCL 70

Query: 71  ACSGCITSAETVMLEKQSLGDFITRINSDKAVIVSVSPQSRASLAAFFGLSQSQVFRKLT 130
           ACSGCITSAETVMLEKQSLGDFITRINSDKAVIVSVSPQSRASLAAFFGLSQSQVFRKLT
Sbjct: 71  ACSGCITSAETVMLEKQSLGDFITRINSDKAVIVSVSPQSRASLAAFFGLSQSQVFRKLT 130

Query: 131 ALFKSMGVKAVYDTSSSRDLSLIEACSEFVTRYHQNQLSSGKEAGKNLPMLSSACPGWIC 190
           ALFKSMGVKAVYDTSSSRDLSLIEACSEFVTRYHQNQLSSGKEAGKNLPMLSSACPGWIC
Sbjct: 131 ALFKSMGVKAVYDTSSSRDLSLIEACSEFVTRYHQNQLSSGKEAGKNLPMLSSACPGWIC 190

Query: 191 YAEKTLGSFILPYISAVKSPQQAIGAAIKHHMVGKLGLKPHDVYHVTVMPCYDKKLEAVR 250
           YAEKTLGSFILPYISAVKSPQQAIGAAIKHHMVGKLGLKPHDVYHVTVMPCYDKKLEAVR
Sbjct: 191 YAEKTLGSFILPYISAVKSPQQAIGAAIKHHMVGKLGLKPHDVYHVTVMPCYDKKLEAVR 250

Query: 251 DDFVFSVEDKDVTEVDSVLTTGEVLDLIQSRSVDFKTLEESPMDRLLTNVDDDGQLXXXX 310
           DDFVFSVEDKDVTEVDSVLTTGEVLDLIQSRSVDFKTLEESPMDRLLTNVDDDGQL    
Sbjct: 251 DDFVFSVEDKDVTEVDSVLTTGEVLDLIQSRSVDFKTLEESPMDRLLTNVDDDGQLYGVS 310

Query: 311 XXXXXXAETVFRHAAHVLFDRKIEGSVDFRILRNSDFREVTLEVEGKPVLKFALCYGFRN 370
                 AETVFRHAAHVLFDRKIEGSVDFRILRNSDFREVTLEVEGKPVLKFALCYGFRN
Sbjct: 311 GGSGGYAETVFRHAAHVLFDRKIEGSVDFRILRNSDFREVTLEVEGKPVLKFALCYGFRN 370

Query: 371 LQNIIRKIKMGKCEYHFIEVMACPSGCLNGGGQIKPAKGQSAKDLIQLLEDVYIQDVSVS 430
           LQNIIRKIKMGKCEYHFIEVMACPSGCLNGGGQIKPAKGQSAKDLIQLLEDVYIQDVSVS
Sbjct: 371 LQNIIRKIKMGKCEYHFIEVMACPSGCLNGGGQIKPAKGQSAKDLIQLLEDVYIQDVSVS 430

Query: 431 NPFENPIAKRLYDEWLGQPGSENAKKYLHTKYHPVVKSVASQLQNW 476
           NPFENPIAKRLYDEWLGQPGSENAKKYLHTKYHPVVKSVASQLQNW
Sbjct: 431 NPFENPIAKRLYDEWLGQPGSENAKKYLHTKYHPVVKSVASQLQNW 476



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 14,392,388
Number of extensions: 556773
Number of successful extensions: 1187
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 1187
Number of HSP's successfully gapped: 1
Length of query: 476
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 371
Effective length of database: 11,553,331
Effective search space: 4286285801
Effective search space used: 4286285801
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)