BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0728500 Os03g0728500|AK101820
         (472 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0728500  SMAD/FHA domain containing protein                  469   e-132

>Os03g0728500 SMAD/FHA domain containing protein
          Length = 472

 Score =  469 bits (1208), Expect = e-132,   Method: Compositional matrix adjust.
 Identities = 271/472 (57%), Positives = 271/472 (57%)

Query: 1   MADAPPPVLTLQVKKGRRGGETRQWRAGAVLRVGRVATGNDIAVRDAGASQRHLSIEFLP 60
           MADAPPPVLTLQVKKGRRGGETRQWRAGAVLRVGRVATGNDIAVRDAGASQRHLSIEFLP
Sbjct: 1   MADAPPPVLTLQVKKGRRGGETRQWRAGAVLRVGRVATGNDIAVRDAGASQRHLSIEFLP 60

Query: 61  PPASRWAVSDVGSSNGTLLNGXXXXXXXXXXXXDGDVIKIGESSMLVVSIAPDSDPNPGP 120
           PPASRWAVSDVGSSNGTLLNG            DGDVIKIGESSMLVVSIAPDSDPNPGP
Sbjct: 61  PPASRWAVSDVGSSNGTLLNGSPLVPTVPSPLSDGDVIKIGESSMLVVSIAPDSDPNPGP 120

Query: 121 RRSSRQSAAVVGEQEKPPAVTRRGGRKNXXXXXXXXXXXXXXXXXXXXXXXXXXTRRGAR 180
           RRSSRQSAAVVGEQEKPPAVTRRGGRKN                          TRRGAR
Sbjct: 121 RRSSRQSAAVVGEQEKPPAVTRRGGRKNAAAAAVAVEPPIAEKEEPEPEEAPVVTRRGAR 180

Query: 181 KKAAQPPKXXXXXXXXXXXXXXXXTRRCGRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           KKAAQPPK                TRRCGR                              
Sbjct: 181 KKAAQPPKAEEHEEGEEEVVAAVVTRRCGRKKAAEPPKPDEEEEQEKGKDEEQEEKEEEE 240

Query: 241 VPVVTRRGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDTSLDERESEMAGKGRGRATR 300
           VPVVTRRG                              VDTSLDERESEMAGKGRGRATR
Sbjct: 241 VPVVTRRGRSRKAAPEAAVAPPPPRARSTRAAARRGKAVDTSLDERESEMAGKGRGRATR 300

Query: 301 SNARKCRMAVPXXXXXXXXXXXXATAVAEEQIKDQPRAMAVTDGEEEDDKVEAMDGEVEQ 360
           SNARKCRMAVP            ATAVAEEQIKDQPRAMAVTDGEEEDDKVEAMDGEVEQ
Sbjct: 301 SNARKCRMAVPEDDDDDGEQQEGATAVAEEQIKDQPRAMAVTDGEEEDDKVEAMDGEVEQ 360

Query: 361 NDKASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420
           NDKAS                                                       
Sbjct: 361 NDKASEEEEVPVARRGRARRAPKGKATASSNAHAASDNAVEEEDGGRGEGAAVEVEGESS 420

Query: 421 XXXXLETMTLREWFQRMNVYLPRMINXXXXXXXXXXXXXXXXIDEYISTLED 472
               LETMTLREWFQRMNVYLPRMIN                IDEYISTLED
Sbjct: 421 GSSSLETMTLREWFQRMNVYLPRMINEAAEEALSALRERHRRIDEYISTLED 472



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.130    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,168,633
Number of extensions: 309487
Number of successful extensions: 1100
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 1095
Number of HSP's successfully gapped: 1
Length of query: 472
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 367
Effective length of database: 11,553,331
Effective search space: 4240072477
Effective search space used: 4240072477
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 158 (65.5 bits)