BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0698900 Os03g0698900|AK073303
         (257 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0698900  Alkaline phytoceramidase family protein             512   e-145

>Os03g0698900 Alkaline phytoceramidase family protein
          Length = 257

 Score =  512 bits (1319), Expect = e-145,   Method: Compositional matrix adjust.
 Identities = 245/257 (95%), Positives = 245/257 (95%)

Query: 1   MADSMVSSFWGPVTSTTELCEENYAHSSYIAEFYNTVSNVPCXXXXXXXXXXXXRQGFEK 60
           MADSMVSSFWGPVTSTTELCEENYAHSSYIAEFYNTVSNVPC            RQGFEK
Sbjct: 1   MADSMVSSFWGPVTSTTELCEENYAHSSYIAEFYNTVSNVPCVLLALVGLVNALRQGFEK 60

Query: 61  RFSVLHISNMILAIGSMIFHATLQHVLQQSDETPMVWEILLYLYVLYSPDWHYRSTMPTF 120
           RFSVLHISNMILAIGSMIFHATLQHVLQQSDETPMVWEILLYLYVLYSPDWHYRSTMPTF
Sbjct: 61  RFSVLHISNMILAIGSMIFHATLQHVLQQSDETPMVWEILLYLYVLYSPDWHYRSTMPTF 120

Query: 121 LFLYGAAFAVVHFLVRFQVVFKLHYVGLCLLCIPRMYKYYIQTKDMAAKRLAKLWVLTLS 180
           LFLYGAAFAVVHFLVRFQVVFKLHYVGLCLLCIPRMYKYYIQTKDMAAKRLAKLWVLTLS
Sbjct: 121 LFLYGAAFAVVHFLVRFQVVFKLHYVGLCLLCIPRMYKYYIQTKDMAAKRLAKLWVLTLS 180

Query: 181 LATLCWLFDRMFCKKLSHWYVNPQGHAWWHILMGFNSYFANTFLMFCRAQQRGWEPKITH 240
           LATLCWLFDRMFCKKLSHWYVNPQGHAWWHILMGFNSYFANTFLMFCRAQQRGWEPKITH
Sbjct: 181 LATLCWLFDRMFCKKLSHWYVNPQGHAWWHILMGFNSYFANTFLMFCRAQQRGWEPKITH 240

Query: 241 LFGFLPYVKIQKPQKRE 257
           LFGFLPYVKIQKPQKRE
Sbjct: 241 LFGFLPYVKIQKPQKRE 257



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.330    0.139    0.473 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,768,662
Number of extensions: 281166
Number of successful extensions: 635
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 635
Number of HSP's successfully gapped: 1
Length of query: 257
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 158
Effective length of database: 11,866,615
Effective search space: 1874925170
Effective search space used: 1874925170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 155 (64.3 bits)