BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0668900 Os03g0668900|AK108369
         (272 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0668900  Conserved hypothetical protein                      258   2e-69
AK106786                                                          120   1e-27

>Os03g0668900 Conserved hypothetical protein
          Length = 272

 Score =  258 bits (660), Expect = 2e-69,   Method: Compositional matrix adjust.
 Identities = 132/153 (86%), Positives = 132/153 (86%)

Query: 22  VKNAPFSAPKTLTLDGAVKSTTGRLPSPSLLGRYPSLEELSVAGXXXXXXXXXXXXXXXX 81
           VKNAPFSAPKTLTLDGAVKSTTGRLPSPSLLGRYPSLEELSVAG                
Sbjct: 22  VKNAPFSAPKTLTLDGAVKSTTGRLPSPSLLGRYPSLEELSVAGARLSSLAGLPRLPALR 81

Query: 82  XXXXXDNRLSGAASLAAVAESCGATLRHLDLGNNRFADVAELAPLAPHGVESLDLYQCPV 141
                DNRLSGAASLAAVAESCGATLRHLDLGNNRFADVAELAPLAPHGVESLDLYQCPV
Sbjct: 82  RLSLPDNRLSGAASLAAVAESCGATLRHLDLGNNRFADVAELAPLAPHGVESLDLYQCPV 141

Query: 142 TKAKGYRDKVFALIPSLKFLDGMDAEGNDCLDS 174
           TKAKGYRDKVFALIPSLKFLDGMDAEGNDCLDS
Sbjct: 142 TKAKGYRDKVFALIPSLKFLDGMDAEGNDCLDS 174



>AK106786 
          Length = 450

 Score =  120 bits (301), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 67/143 (46%), Positives = 87/143 (60%), Gaps = 2/143 (1%)

Query: 28  SAPKTLTLDGAVKSTTGRLPSPSLLGRYPSLEELSVAGXXXXXXXXXXXXXXXXXXXXXD 87
           S  ++LTLDGAVK   GRLP+  +L RY SLE LS+AG                     D
Sbjct: 22  SPARSLTLDGAVKCLHGRLPAAEILERYQSLEHLSIAGVGVASLAGFPRLRNLTRLTLSD 81

Query: 88  NRLSGAASLAAVAESCGATLRHLDLGNNRFADVAELAPLAPHGVESLDLYQCPVTKAKGY 147
           NR++G   L  +  +  A+LR LDL NNR  DV +L+PLA   + SLDLY+CPVT+ K Y
Sbjct: 82  NRIAGG--LDHLVAAGLASLRDLDLSNNRIQDVGDLSPLANLRLVSLDLYECPVTRVKDY 139

Query: 148 RDKVFALIPSLKFLDGMDAEGND 170
           R KVF +I +LK+LD MDA+ N+
Sbjct: 140 RSKVFGMIRTLKYLDKMDADENE 162



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.311    0.129    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,759,334
Number of extensions: 176455
Number of successful extensions: 488
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 486
Number of HSP's successfully gapped: 3
Length of query: 272
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 173
Effective length of database: 11,866,615
Effective search space: 2052924395
Effective search space used: 2052924395
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 155 (64.3 bits)