BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0381500 Os03g0381500|AK108125
         (199 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0381500  Conserved hypothetical protein                      174   4e-44

>Os03g0381500 Conserved hypothetical protein
          Length = 199

 Score =  174 bits (440), Expect = 4e-44,   Method: Compositional matrix adjust.
 Identities = 94/148 (63%), Positives = 94/148 (63%)

Query: 52  FLMSPQPGGGKPPVXXXXXXXXXXXXXXXXXXXXXXXXAEPVEQAVGPGAAAIPGFNSRL 111
           FLMSPQPGGGKPPV                        AEPVEQAVGPGAAAIPGFNSRL
Sbjct: 52  FLMSPQPGGGKPPVEGVSGGGDSDGTRGGGGGLEGVKGAEPVEQAVGPGAAAIPGFNSRL 111

Query: 112 DAFRAWAKLTWMKLRRPHSGEPRRYXXXXXXXXXXXXXXKRSLEMTKETVEQXXXXXXXX 171
           DAFRAWAKLTWMKLRRPHSGEPRRY              KRSLEMTKETVEQ        
Sbjct: 112 DAFRAWAKLTWMKLRRPHSGEPRRYDDDAGSSGSAADAAKRSLEMTKETVEQAAASAARA 171

Query: 172 XXXXXXXXSDKVKGAASPAKRAPSDAEL 199
                   SDKVKGAASPAKRAPSDAEL
Sbjct: 172 AGNAVGKASDKVKGAASPAKRAPSDAEL 199



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.311    0.127    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,216,471
Number of extensions: 108871
Number of successful extensions: 176
Number of sequences better than 1.0e-10: 1
Number of HSP's gapped: 174
Number of HSP's successfully gapped: 1
Length of query: 199
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 103
Effective length of database: 12,023,257
Effective search space: 1238395471
Effective search space used: 1238395471
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 153 (63.5 bits)