BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0305500 Os03g0305500|AK070638
         (519 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0305500  Argininosuccinate lyase domain containing protein  1031   0.0  
Os03g0824700                                                      141   1e-33
Os03g0825000                                                      139   6e-33

>Os03g0305500 Argininosuccinate lyase domain containing protein
          Length = 519

 Score = 1031 bits (2665), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 503/519 (96%), Positives = 503/519 (96%)

Query: 1   MAAATSQSFLSPAPNPLLRPRILPFPAGGSVSLRGRRPAFPXXXXXXXXXXXXXXXXRKE 60
           MAAATSQSFLSPAPNPLLRPRILPFPAGGSVSLRGRRPAFP                RKE
Sbjct: 1   MAAATSQSFLSPAPNPLLRPRILPFPAGGSVSLRGRRPAFPSVAAASTSMASSESEERKE 60

Query: 61  TKLWGGRFEEGVTDAVEGFTESISYDWQLYKYDIMGSKAHASMLAAQGLITAGDKDIILE 120
           TKLWGGRFEEGVTDAVEGFTESISYDWQLYKYDIMGSKAHASMLAAQGLITAGDKDIILE
Sbjct: 61  TKLWGGRFEEGVTDAVEGFTESISYDWQLYKYDIMGSKAHASMLAAQGLITAGDKDIILE 120

Query: 121 GLDQIEKLIQDGKFEWRTDREDVHMNIEAALIEKVGEPAKKLHTARSRNDQIVTDLRLWC 180
           GLDQIEKLIQDGKFEWRTDREDVHMNIEAALIEKVGEPAKKLHTARSRNDQIVTDLRLWC
Sbjct: 121 GLDQIEKLIQDGKFEWRTDREDVHMNIEAALIEKVGEPAKKLHTARSRNDQIVTDLRLWC 180

Query: 181 RDAIDKILFRIKQFQVSLVLLASKYVDLIVPGYTHLQRAQPVLLPHLLLSYVEQLERDAG 240
           RDAIDKILFRIKQFQVSLVLLASKYVDLIVPGYTHLQRAQPVLLPHLLLSYVEQLERDAG
Sbjct: 181 RDAIDKILFRIKQFQVSLVLLASKYVDLIVPGYTHLQRAQPVLLPHLLLSYVEQLERDAG 240

Query: 241 RLVNCRERLNFCPLGACALAGTGLPIDRFKTAKDLKFTAPMKNSIDAVSDRDFVLEFLAA 300
           RLVNCRERLNFCPLGACALAGTGLPIDRFKTAKDLKFTAPMKNSIDAVSDRDFVLEFLAA
Sbjct: 241 RLVNCRERLNFCPLGACALAGTGLPIDRFKTAKDLKFTAPMKNSIDAVSDRDFVLEFLAA 300

Query: 301 NSIAAVHLSRIGEEWVLWASEEFGFLTPSDSVSTGSSIMPQKKNPDPMELVRGKSARVFG 360
           NSIAAVHLSRIGEEWVLWASEEFGFLTPSDSVSTGSSIMPQKKNPDPMELVRGKSARVFG
Sbjct: 301 NSIAAVHLSRIGEEWVLWASEEFGFLTPSDSVSTGSSIMPQKKNPDPMELVRGKSARVFG 360

Query: 361 DLMTVLTLCKGLPQAYNRDLQEDKEPLFDSVKAVLGMLEVCTEFAQNISFNSKRIQSSLP 420
           DLMTVLTLCKGLPQAYNRDLQEDKEPLFDSVKAVLGMLEVCTEFAQNISFNSKRIQSSLP
Sbjct: 361 DLMTVLTLCKGLPQAYNRDLQEDKEPLFDSVKAVLGMLEVCTEFAQNISFNSKRIQSSLP 420

Query: 421 AGYLDATTLADYLVKKGVPFRTSHEIVGRSVALCVSKNCQLAELGLDDLKSVHPVFEGDV 480
           AGYLDATTLADYLVKKGVPFRTSHEIVGRSVALCVSKNCQLAELGLDDLKSVHPVFEGDV
Sbjct: 421 AGYLDATTLADYLVKKGVPFRTSHEIVGRSVALCVSKNCQLAELGLDDLKSVHPVFEGDV 480

Query: 481 YEYLGVENAVNKFISYGSTGSEQVKKQLEDWRTQLGISS 519
           YEYLGVENAVNKFISYGSTGSEQVKKQLEDWRTQLGISS
Sbjct: 481 YEYLGVENAVNKFISYGSTGSEQVKKQLEDWRTQLGISS 519



>Os03g0824700 
          Length = 415

 Score =  141 bits (356), Expect = 1e-33,   Method: Compositional matrix adjust.
 Identities = 128/449 (28%), Positives = 216/449 (48%), Gaps = 56/449 (12%)

Query: 73  TDAVEGFTESISYDWQLYKYDIMGSKAHASMLAAQGLITAGDKDIILEGLDQIEKLIQDG 132
           T+  E F +SI +D  LYKY I+G K +A  +  +G+I++ +++ I+  L++IE  I+ G
Sbjct: 11  TNFDESFVDSIVFDKTLYKYAILGCKVNADGIMNEGIISSSERNKIIHALERIEDDIEIG 70

Query: 133 KFEWRTDREDVHMNIEAALIEKVGEPAKKLHTARSRNDQIVTDLRLWCRDAIDKILFRIK 192
           KF+WR D  DVH +I  AL + +G+ AK L    S+ D  +  L  W +++ID I+ ++K
Sbjct: 71  KFKWR-DGADVHTSIVEALADMIGDQAKGL-AVESKCDSCLMILETWSKNSIDHIMTQLK 128

Query: 193 QFQVSLVLLASKYVDLIVPGYTHLQRAQPVLLPHLLLSYVEQLERDAGRLVNCRERLNFC 252
           Q Q +LVLLA K    ++PG   ++         LL+  V+ L+ DA +L NC      C
Sbjct: 129 QLQAALVLLAIKNDGFVLPGEKEIEGTS------LLMRIVKALDSDASKLRNCLG--GIC 180

Query: 253 PLGACALAGTGLPIDRFKTAKDLKFTAPMKNSIDAVSDRDFVLEFLAANSIAAVHLSRIG 312
                       P D   T   L + A   NSI      ++ +  L    +  V LS   
Sbjct: 181 STDGVIFMRLSSPEDY--TLSKLSYIAWFANSI------NYRIPNLLRQLLEKV-LSLRN 231

Query: 313 EEWVLWASEEFGFLTPSDSVSTGSSIMPQKKNPDPMELVRGKSARVFGDLMTVLTLCKGL 372
            E  ++ +          ++   SSI   +++ D + + +  + R FG     +  C   
Sbjct: 232 AETAIYET----------TLGKLSSIEQMRRHGD-VAISKCLNLR-FGKACGSVQTC--- 276

Query: 373 PQAYNRDLQEDKEPLFDSVKAVLGMLEVCTEFAQNISFNSKRIQSSLPAGYLDATTLADY 432
                 ++++ K  LF S K+V+ +L++  +  ++ISF+ ++ Q+ LP GY D    A +
Sbjct: 277 ------EIEDAKHHLFSSTKSVVEILDLSIQLVKSISFDMEKAQNCLPRGYDDIMRFAHF 330

Query: 433 LVKKGVPFRTSHEIVGRSVALCVSKNCQLAELGLD--DLKSVHPVFEGDVY--EYLGVEN 488
           L  KG+   T++ +    V LC+ K  Q +EL L+  +LK +    E   Y  EY G   
Sbjct: 331 LTTKGIDSVTAYAL----VHLCLDKQLQPSELTLEKRELKQIDFHCERAHYLLEYKGG-- 384

Query: 489 AVNKFISYGSTGSEQVKKQLEDWRTQLGI 517
                  +G +      KQ+  W ++L I
Sbjct: 385 ------IFGDSTDLDACKQMLKWCSKLRI 407



>Os03g0825000 
          Length = 792

 Score =  139 bits (349), Expect = 6e-33,   Method: Compositional matrix adjust.
 Identities = 124/441 (28%), Positives = 208/441 (47%), Gaps = 53/441 (12%)

Query: 58  RKETKLW-------GGRFEEGVTDAVEG-FTESISYDWQLYKYDIMGSKAHASMLAAQGL 109
           +KE K W         R+ + V  + +  F +SI +D  LYKY I+G K +A  +  QG+
Sbjct: 354 KKEHKGWMQFRSEMNSRYNKAVDISFDDRFADSIVFDKTLYKYAILGCKVNADGIMNQGV 413

Query: 110 ITAGDKDIILEGLDQIEKLIQDGKFEWRTDREDVHMNIEAALIEKVGEPAKKLHTARSRN 169
           I+A +++ I+  L++IE  I+ GKF+WR D  D+H +I  AL + +G+ AK L    S+ 
Sbjct: 414 ISASERNKIIHALERIEGDIEIGKFKWR-DGADIHTSIVEALADMIGDKAKDL-AVESKY 471

Query: 170 DQIVTDLRLWCRDAIDKILFRIKQFQVSLVLLASKYVDLIVPGYTHLQRAQPVLLPHLLL 229
           D  +  L  W +++ID I+ ++KQ QV+LVLLA K  D ++ G   ++      L  LLL
Sbjct: 472 DSCLMILETWSKNSIDHIMTQLKQLQVALVLLAIKN-DGVILGEREIE--GTTCLESLLL 528

Query: 230 SYVEQLERDAGRLVNCRERLNFCPLGACALAGTGLPIDRFKTAKDLKFTAPMKNSIDAVS 289
             V+ L+ DA +L +    ++ C      L  T  P ++  +   L   A    SI    
Sbjct: 529 RIVKVLDYDASKLRSYHGSIS-CSNDGVMLLRTSYPENQMFS--KLSSMASFAESI---- 581

Query: 290 DRDFVLEFLAANSIAAVHLSRIGEEWVLWASEEFGFLTPSDSVSTGSSIMPQKKNPDPME 349
                      N+    HL ++ E+  + +      LTP+   +   +++ +  + +   
Sbjct: 582 -----------NNCIPNHLRQLLEK--VLSRRNILLLTPNGETTIYDTVLSKFGSIE--- 625

Query: 350 LVRGKSARVFGDLMTVLTLCKGL------PQAYNRDLQEDKEPLFDSVKAVLGMLEVCTE 403
                  R  GD+     L  G       PQ    ++++ K  LF S K+V+ +L++  +
Sbjct: 626 -----QTRHHGDVAISKCLNIGFRNVRESPQTC--EIEDAKHYLFSSTKSVVEILDLSIQ 678

Query: 404 FAQNISFNSKRIQSSLPAGYLDATTLADYLVKKGVPFRTSHEIVGRSVALCVSKNCQLAE 463
               I F  ++ Q+SLP GY D      +L  KG+   T++ +    V LC+ K  Q +E
Sbjct: 679 LVLRILFGMEKAQNSLPRGYHDVMRFTHFLTTKGIDLGTAYAL----VHLCLDKKLQPSE 734

Query: 464 LGLDDLKSVHPVFEGDVYEYL 484
           L LD  +     F  +   YL
Sbjct: 735 LILDKHELKQIDFHCERAHYL 755



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 16,920,850
Number of extensions: 691451
Number of successful extensions: 1629
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1624
Number of HSP's successfully gapped: 3
Length of query: 519
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 414
Effective length of database: 11,553,331
Effective search space: 4783079034
Effective search space used: 4783079034
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)