BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0254800 Os03g0254800|AK099850
         (438 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0254800  Similar to Chorismate synthase 1, chloroplast ...   858   0.0  
AB122084                                                          360   1e-99

>Os03g0254800 Similar to Chorismate synthase 1, chloroplast precursor (EC
           4.2.3.5) (5- enolpyruvylshikimate-3-phosphate
           phospholyase 1)
          Length = 438

 Score =  858 bits (2216), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 420/438 (95%), Positives = 420/438 (95%)

Query: 1   MAAPTSSQPVARVLPRXXXXXFRAFPESAPASLRFSVGRRRAARLEVKASANVFGNYFQV 60
           MAAPTSSQPVARVLPR     FRAFPESAPASLRFSVGRRRAARLEVKASANVFGNYFQV
Sbjct: 1   MAAPTSSQPVARVLPRGGGGGFRAFPESAPASLRFSVGRRRAARLEVKASANVFGNYFQV 60

Query: 61  ATYGESHGGGVGCVISGCPPRIPLTEADMQVELDRRRPGQSRITTPRKETDTCKILSGTH 120
           ATYGESHGGGVGCVISGCPPRIPLTEADMQVELDRRRPGQSRITTPRKETDTCKILSGTH
Sbjct: 61  ATYGESHGGGVGCVISGCPPRIPLTEADMQVELDRRRPGQSRITTPRKETDTCKILSGTH 120

Query: 121 EGMTTGTPIHVFVPNTDQRGGDYSEMAKAYRPSHADATYDFKYGVRAVQGGGRSSARETI 180
           EGMTTGTPIHVFVPNTDQRGGDYSEMAKAYRPSHADATYDFKYGVRAVQGGGRSSARETI
Sbjct: 121 EGMTTGTPIHVFVPNTDQRGGDYSEMAKAYRPSHADATYDFKYGVRAVQGGGRSSARETI 180

Query: 181 GRVXXXXXXXXXXXXXSGVEILAFVSKVHQVVLPEDAVDYDTVTMEQIESNIVRCPDPEY 240
           GRV             SGVEILAFVSKVHQVVLPEDAVDYDTVTMEQIESNIVRCPDPEY
Sbjct: 181 GRVAAGALAKKILKLKSGVEILAFVSKVHQVVLPEDAVDYDTVTMEQIESNIVRCPDPEY 240

Query: 241 AQKMIDAIDKVRVRGDSIGGVVTCIARNVPRGIGSPVFDKLEAELAKAMLSLPASKGFEI 300
           AQKMIDAIDKVRVRGDSIGGVVTCIARNVPRGIGSPVFDKLEAELAKAMLSLPASKGFEI
Sbjct: 241 AQKMIDAIDKVRVRGDSIGGVVTCIARNVPRGIGSPVFDKLEAELAKAMLSLPASKGFEI 300

Query: 301 GSGFAGTDYTGSEHNDEFYMDEAGNVRTRTNRSGGVQGGISNGEIIYFKVAFKPTATIGK 360
           GSGFAGTDYTGSEHNDEFYMDEAGNVRTRTNRSGGVQGGISNGEIIYFKVAFKPTATIGK
Sbjct: 301 GSGFAGTDYTGSEHNDEFYMDEAGNVRTRTNRSGGVQGGISNGEIIYFKVAFKPTATIGK 360

Query: 361 KQHTVSREHEDVELLARGRHDPCVVPRAVPMVESMAALVLMDQLMAHIAQCEMFPLNLAL 420
           KQHTVSREHEDVELLARGRHDPCVVPRAVPMVESMAALVLMDQLMAHIAQCEMFPLNLAL
Sbjct: 361 KQHTVSREHEDVELLARGRHDPCVVPRAVPMVESMAALVLMDQLMAHIAQCEMFPLNLAL 420

Query: 421 QEPVGSASSVPAFAPDLS 438
           QEPVGSASSVPAFAPDLS
Sbjct: 421 QEPVGSASSVPAFAPDLS 438



>AB122084 
          Length = 219

 Score =  360 bits (924), Expect = 1e-99,   Method: Compositional matrix adjust.
 Identities = 171/219 (78%), Positives = 191/219 (87%)

Query: 65  ESHGGGVGCVISGCPPRIPLTEADMQVELDRRRPGQSRITTPRKETDTCKILSGTHEGMT 124
           ESHGGGVGC+I GCPPRIPL+EADMQ +LDRRRPGQSRITTPRKETDTCKI SG  EG+T
Sbjct: 1   ESHGGGVGCIIDGCPPRIPLSEADMQFDLDRRRPGQSRITTPRKETDTCKISSGVSEGLT 60

Query: 125 TGTPIHVFVPNTDQRGGDYSEMAKAYRPSHADATYDFKYGVRAVQGGGRSSARETIGRVX 184
           TGTPIHVFVPNTDQRG DYSEM+ AYRPSHADATYD KYGVR+VQGGGRSSARETIGRV 
Sbjct: 61  TGTPIHVFVPNTDQRGLDYSEMSVAYRPSHADATYDMKYGVRSVQGGGRSSARETIGRVA 120

Query: 185 XXXXXXXXXXXXSGVEILAFVSKVHQVVLPEDAVDYDTVTMEQIESNIVRCPDPEYAQKM 244
                       +G EILA+VS+VH+VVLPE  VD+D++T++Q+ESNIVRCPDPEYA+KM
Sbjct: 121 AGGVAKXILKLYAGTEILAYVSQVHKVVLPEGVVDHDSLTLDQMESNIVRCPDPEYAEKM 180

Query: 245 IDAIDKVRVRGDSIGGVVTCIARNVPRGIGSPVFDKLEA 283
           I AID VRV+GDS+GGVVTCI RN PRG+G PVFDKLEA
Sbjct: 181 IAAIDAVRVKGDSVGGVVTCIVRNXPRGLGXPVFDKLEA 219



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,116,111
Number of extensions: 666704
Number of successful extensions: 1639
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1639
Number of HSP's successfully gapped: 2
Length of query: 438
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 334
Effective length of database: 11,605,545
Effective search space: 3876252030
Effective search space used: 3876252030
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 157 (65.1 bits)